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Life Science
Microplate Reader
Tristar 5 Multimode Reader
Overview
Flexibility and sensitivity whenever you need it
The Tristar 5 is a modular high-performance microplate reader equipped with independent, user-selectable filters and monochromators on both, the excitation and emission side for any measurement. This guarantees both, flexibility and sensitivity whenever you need it. The system supports advanced detection modes such as TRF and TR-FRET, and FP as well as specific assays like BRET/BRET2, NanoBRET™ , Lantha-Screen® or AlphaScreen®.
Highlights
Detection Technologies
The Tristar 5 Multimode Microplate Reader provides you with application flexibility for today, tomorrow, and beyond to master your changing plate reading applications. The system supports the following wide range of detection modes:
- Absorbance (UV/VIS)
- Fluorescence Intensity (including FRET)
- Luminescence (including BRET and BRET2)
- Time Resolved Fluorescence (TRF)
- Time Resolved FRET (TR-FRET / HTRF®)
- Fluorescence Polarization
- AlphaScreen®
FlexTec Optics – when flexibility AND sensitivity count
The Tristar 5 system is equiped with FlexTec Optics, offering you the best of two worlds – benefit from the flexibility to easily select a discrete wavelength and perform spectral scans for both, excitation and emission using its built-in monochromator technology. Or optimize your assays´ sensitivity by utilizing the system filter sliders for excitation and emission. Or mix both technologies if required: the Tristar 5 delivers both, flexibility and sensitivity in a single system.
The Tristar 5 employs up to two double monochromators providing blocking properties needed in sensitive fluorescence assays. Both monochromators are equipped with software driven continuous bandwidth variation to optimise the instrument for the specific demands of different assay conditions.
Berthold´s flexible monochromator technology offers you variable bandwidths from 4-12 nm in excitation and 8 – 22 nm for emission, selectable in 1 nm increments.
Broad wavelength range selection from UV through the visible range
From UV absorbance for DNA quantification to measurements in the far red or NIR (in selected configurations), the Tristar 5 is ready for any assay.
Advanced bottom reading technology - enhanced cell-based assay performance
The Tristar 5 enables you to read both, from below as well as from the top with high-performance. The advanced bottom-reading technology of the system can be easily selected via the software to help you analyse e.g. adherent cells and obtain best sensitivity and a superior signal-to-background ratio for these applications.
ONE-4-ALL Optics - uncompromised performance
Our patented ONE-4-ALL optics have been optimised to combine the stability and user-friendliness of a multimodal optical system with the sensitivity and versatility of dedicated optical devices.
ONE-4-ALL optics work for both filter- and monochromator-based applications, so there´s never a compromise in performance in any mode.
High-performance filter system - when sensitivity counts
The quick-change filter technology of the Tristar series provides you with the flexibility required to meet your application needs: up to 40 different excitation and emission filters can be easily mounted on exchangeable filter slides.
The filters are characterized by high transmissions properties which can be up to 25-fold that of monochromators. Technologies like Time-Resolved Fluorescence (TRF) can be measured more efficiently with filters.
Furthermore, filters with a large bandwidth are available to analyze fluorophores with wide spectra and all luminescence-based assay requiring to use filters, e.g. BRET, BRET2 or NanoBRET™ assays.
Ergonomic design - supporting your workflows
The ergonomic design of the system provides full front access for all key operations (e.g. plate loading, filter change and connecting reagents). Reagent vials can be stored in an integrated front compartment, providing easy access and visibility. It contains a removable trough that can be filled with water or ice to keep all reagents cooled. Different reagent tube holders are available to ensure secure handling of valuable reagents. The system has a flat surface on its top, providing enough space to put down a laptop.
Powerful crosstalk control – reliable results
Do you often see a few high-intensity samples surrounded by wells of low-intensity when running your samples? Then you´re potentially affected by a phenomenon called crosstalk, that can produce wrong results and, hence, wrong conclusions. The crosstalk reduction design of the Tristar 5 does almost eliminate this problem: with a crosstalk of 10-6, you never have to worry about the position of your high-intensity samples in your plate.
JET injectors: highest accuracy, speed and cell-friendliness
The Tristar 5 can be equipped with up to 3 JET injectors with variable volumes in pre-measurement or measurement position. The ultra-fast injection speed of JET injectors eliminates any delay between injection and measurement and enables measurement of fast reactions, e.g. flash luminescence.
Berthold Technologies' JET injectors use Teflon bellows for accurate and fast injections and guarantee most efficient mixing as well as extreme longevity.
- Highest accuracy and reproducibility - 98 % accuracy over the entire volume range
- Low reagent consumption - Low dead volume. Recovers up to 60 % of the reagent in the injector line. A single priming sequence is sufficient to achieve a 99 % homogeneous mix at the tip.
- Low maintenance costs – friction-free operation enables more than 3 million injections without mechanical failure.
- Optimal performance in flash-kinetics - ultra-fast injection enables the measurement of the first 150 ms of a kinetic assay (e.g. Fura-2 or Acridiniumester-assys).
- Worry-free injection of cell suspensions - negligible shear forces ideal when working with living cells, e.g. in Aequorin-based calcium assays
Convenient Software options
Our microplate readers are powered by LightCompass, a flexible software enabling easy data acquisition, analysis and reporting using Berthold Technologies' instruments. It has been optimized for different microplate and tube formats. LightCompass features
all the functions you can expect from an advanced scientific software, such as flexible creation of measurement
protocols, curve fitting, qualitative analysis and compliance with FDA 21 CFR Part 11.
LightCompass is available in 3 different versions: Basic, Plus and Professional. Just pick the version that better suits your research needs.
| Technical Details | Kindly refer to the "Downloads" tab. |
Authors: Alexandre Pierre, Claire Bourel, Raphael Favory, Benoit Brassart, Frederic Wallet, Frederic N. Daussin, Sylvain Normandin, Michael Howsam, Raphael Romien, Jeremy Lemaire, Gaelle Grolaux, Arthur Durand, Marie Frimat, Bruno Bastide, Philippe Amouyel, Eric Boulanger, Sebastien Preau, and Steve Lancel
Journal: Biology
Sepsis is a life-threatening infection usually resulting in admission to an Intensive Care Unit, and is responsible for 1 in 5 deaths worldwide. Muscle weakness is a common and early complication of sepsis that impairs short- and long-term prognoses. It is characterized by muscle fiber atrophy and mitochondrial dysfunction. In addition, sepsis induces a negative imbalance in energy homeostasis, also associated with hospital complications. However, whether this energy deficit disrupts the metabolism in skeletal muscle has never been studied. Our study compared three groups of mice (sepsis with a spontaneous energy deficit, control without an energy deficit, and control with an energy deficit) to investigate the impact of energy debt on skeletal muscle in the first 24 h of sepsis. We demonstrated that a sepsis-like energy deficit was not in itself sufficient to alter muscle fiber size or the mitochondrial population, in contrast with sepsis. Conversely, an energy deficit led to important metabolic adaptations that were not found in sepsis.
Authors: Tanja Jeblick, Thomas Leisen, Christina E Steidele, Isabell Albert, Jonas Müller, Sabrina Kaiser, Florian Mahler, Frederik Sommer, Sandro Keller, Ralph Hückelhoven, Matthias Hahn, and David Scheuring
Journal: Plant Physiology
According to their lifestyle, plant pathogens are divided into biotrophic and necrotrophic organisms. Biotrophic pathogens exclusively nourish living host cells, whereas necrotrophic pathogens rapidly kill host cells and nourish cell walls and cell contents. To this end, the necrotrophic fungus Botrytis cinerea secretes large amounts of phytotoxic proteins and cell wall-degrading enzymes. However, the precise role of these proteins during infection is unknown. Here, we report on the identification and characterization of the previously unknown toxic protein hypersensitive response-inducing protein 1 (Hip1), which induces plant cell death. We found the adoption of a structurally conserved folded Alternaria alternata Alt a 1 protein structure to be a prerequisite for Hip1 to exert its necrosis-inducing activity in a host-specific manner. Localization and the induction of typical plant defense responses by Hip1 indicate recognition as a pathogen-associated molecular pattern at the plant plasma membrane. In contrast to other secreted toxic Botrytis proteins, the activity of Hip1 does not depend on the presence of the receptor-associated kinases BRI1-associated kinase 1 and suppressor of BIR1-1. Our results demonstrate that recognition of Hip1, even in the absence of obvious enzymatic or pore-forming activity, induces strong plant defense reactions eventually leading to plant cell death. Botrytis hip1 overexpression strains generated by CRISPR/Cas9 displayed enhanced infection, indicating the virulence-promoting potential of Hip1. Taken together, Hip1 induces a noncanonical defense response which might be a common feature of structurally conserved fungal proteins from the Alt a 1 family.
Authors: Lin Liu, Wenjuan Lu, Jiahui Dong, Yongya Wu, Meng Tang, Geyu Lianga and Lu Kong
Journal: Nanoscale
Male reproductive health is deteriorating, and fertility is largely affected by environmental factors. This study aims to investigate the potential mechanism underlying mitochondrial division and mitochondrial autophagy in the male reproductive toxicity of nickel nanoparticles (Ni NPs). An in vivo mouse (BALB/c) model was constructed to calculate testicular organ coefficients and sperm abnormality rates, and detect serum reproductive hormones, testicular pathological morphology, and the expression of Drp1, Pink1, and Parkin proteins. Furthermore, mouse spermatogonia (GC-1 cells) were used as an in vitro model to detect cell viability, apoptosis, intracellular reactive oxygen species (ROS), mitochondrial membrane potential (MMP), ATP and protein expression. After treatment with an additional inhibitor, Mdivi-1, such influences were further detected to explore the possible mechanism of male reproductive toxicity induced by Ni NPs. The in vivo studies showed that compared with the control group, exposure to Ni NPs reduced the serum levels of testosterone, follicle stimulating hormone and luteinizing hormone, increased the sperm abnormality rate, widened the gaps in the seminiferous tubules of the testes, decreased the sperm count, and increased the expression of Drp1, Pink1 and Parkin proteins (all P < 0.05). The in vitro studies further confirmed that compared with the control group, Ni NPs can lead to decreased cell viability, increased apoptosis, accumulation of ROS, decreased MMP and ATP, increased expression of Drp1, Pink1, Parkin, Bax, caspase-9 and caspase-3 proteins, and decreased expression of Bcl-2, resulting in an increased value of Bax/Bcl-2. It is worth noting that such influences induced by Ni NPs were significantly reversed by the additional Mdivi-1. In conclusion, Drp1-mediated mitochondrial division and Pink1/Parkin-mediated mitochondrial autophagy play an important role in the male reproductive toxicity of Ni NPs, during which both of them form an interaction cycle and accelerate the occurrence of cell apoptosis.