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Life Science
Microplate Reader
Tristar 3 Multimode Reader
Overview
Simplicity and sensitivity for all levels of experience
The Tristar 3 is a user-friendly and affordable filter-based multimode plate reader that offers the high-performance analysis you expect from Berthold Technologies instruments. Equipped with ONE-4-ALL optics for uncompromised performance of all detection modes, the system is ready to perform absorbance, luminescence and fluorescence measurements.
Highlights
Detection Technologies
The Tristar 3 Multimode Microplate Reader provides you with application flexibility for today, tomorrow, and beyond to master your changing plate reading applications:
- High-sensitivity> Luminescence
- BRET
- Absorbance (UV/VIS)
- Fluorescence
- FRET
Broad wavelength range selection from UV through the visible range
From UV absorbance for DNA quantification to measurements in the far red or NIR (in selected configurations), the Tristar 3 is ready for any assay and greatly improves the wavelength range of the older TriStar².
BRET/BRET2 and NanoBRET™ upgradable
All Tristar 3 preconfigured models have the features you need to perform BRET assays: best-in-class luminescence sensitivity and a high quality filter system. To start working with BRET, just order the upgrade for your specific assay: BRET1, BRET2, NanoBRET™ or others.
ONE-4-ALL Optics for uncompromised performance
Our patented ONE-4-ALL optics have been optimised to combine the stability and user-friendliness of a multimodal optical system with the sensitivity and versatility of dedicated optical devices.
ONE-4-ALL optics work for both filter- and monochromator-based applications, so there´s never a compromise in performance in any mode.
High-performance filter system - when sensitivity counts
The quick-change filter technology of the Tristar series provides you with the flexibility required to meet your application needs: up to 40 different excitation and emission filters can be easily mounted on exchangeable filter slides.
The filters are characterized by high transmissions properties which can be up to 25-fold that of monochromators. Technologies like Time-Resolved Fluorescence (TRF) can be measured more efficiently with filters.
Furthermore, filters with a large bandwidth are available to analyze fluorophores with wide spectra and all luminescence-based assay requiring to use filters, e.g. BRET, BRET2 and NanoBRET™ assays.
Ergonomic design to support your work
The ergonomic design of the system provides full front access for all key operations (e.g. plate loading, filter change and connecting reagents). Reagent vials can be stored in an integrated front compartment, providing easy access and visibility. It contains a removable trough that can be filled with water or ice to keep all reagents cooled. Different reagent tube holders are available to ensure secure handling of valuable reagents. The system has a flat surface on its top, providing enough space to put down a laptop.
Powerful crosstalk control – reliable results
Do you often see a few high-intensity samples surrounded by wells of low-intensity when running your samples? Then you´re potentially affected by a phenomenon called crosstalk, that can produce wrong results and, hence, wrong conclusions. The crosstalk reduction design of the Tristar 3 does almost eliminate this problem: with a crosstalk of 10-6, you never have to worry about the position of your high-intensity samples in your plate.
JET injectors: highest accuracy, speed and cell-friendliness
The Tristar 3 can be equipped with up to 3 JET injectors with variable volumes in pre-measurement or measurement position. The ultra-fast injection speed of JET injectors eliminates any delay between injection and measurement and enables measurement of fast reactions, e.g. flash luminescence.
Berthold Technologies' JET injectors use Teflon bellows for accurate and fast injections and guarantee most efficient mixing as well as extreme longevity.
- Highest accuracy and reproducibility - 98 % accuracy over the entire volume range
- Low reagent consumption - Low dead volume. Recovers up to 60 % of the reagent in the injector line. A single priming sequence is sufficient to achieve a 99 % homogeneous mix at the tip.
- Low maintenance costs – friction-free operation enables more than 3 million injections without mechanical failure.
- Optimal performance in flash-kinetics - ultra-fast injection enables the measurement of the first 150 ms of a kinetic assay (e.g. Fura-2 or Acridiniumester-assys).
- Worry-free injection of cell suspensions - negligible shear forces ideal when working with living cells, e.g. in Aequorin-based calcium assays
Convenient Software options
Our microplate readers are powered by LightCompass, a flexible software enabling easy data acquisition, analysis and reporting using Berthold Technologies' instruments. It has been optimized for different microplate and tube formats. LightCompass features
all the functions you can expect from an advanced scientific software, such as flexible creation of measurement
protocols, curve fitting, qualitative analysis and compliance with FDA 21 CFR Part 11.
LightCompass is available in 3 different versions: Basic, Plus and Professional. Just pick the version that better suits your research needs.
| Technical Details | Kindly refer to the "Downloads" tab. |
Authors: Martin J. Raftery, Alexander Sebastian Franzén, Clarissa Radecke, Abdelhadi Boulifa, Günther Schönrich, Sebastian Stintzing, Jens-Uwe Blohmer, Writing – review & editing, Gabriele Pecher
Journal: International Journal of Molecular Sciences
There is a medical need to develop new and effective therapies against triple-negative breast cancer (TNBC). Chimeric antigen receptor (CAR) natural killer (NK) cells are a promising alternative to CAR-T cell therapy for cancer. A search for a suitable target in TNBC identified CD44v6, an adhesion molecule expressed in lymphomas, leukemias and solid tumors that is implicated in tumorigenesis and metastases. We have developed a next-generation CAR targeting CD44v6 that incorporates IL-15 superagonist and checkpoint inhibitor molecules. We could show that CD44v6 CAR-NK cells demonstrated effective cytotoxicity against TNBC in 3D spheroid models. The IL-15 superagonist was specifically released upon recognition of CD44v6 on TNBC and contributed to the cytotoxic attack. PD1 ligands are upregulated in TNBC and contribute to the immunosuppressive tumor microenvironment (TME). Competitive inhibition of PD1 neutralized inhibition by PD1 ligands expressed on TNBC. In total, CD44v6 CAR-NK cells are resistant to TME immunosuppression and offer a new therapeutic option for the treatment of BC, including TNBC.
Authors: Hannah J. Stage, Susanne Trappe, Katharina Söllig, Dagmar S. Trachsel, Katharina Kirsch, Cornelia Zieger, Roswitha Merle, Jörg R. Aschenbach, Heidrun Gehlen
Journal: Animals
Adult multipotent mesenchymal stem/stromal cells (MSCs) have the ability to self-renew and differentiate into various cell types. MSCs, especially those derived from adipose tissue (ASCs), are easily available and abundant. In order to expand the current knowledge of clinically relevant properties of ASCs obtained from equine abdominal, retrobulbar and subcutaneous adipose tissue using explantation and collagenase digestion, the study’s aim was to investigate the proliferation and multilineage differentiation potential of equine ASCs in vitro. ASCs showed a high proliferation and adipogenic, osteogenic and chondrogenic differentiation potential, whereby a significant effect of the tissue localization and isolation procedure was detected in the case of adipogenic and osteogenic differentiation. However, equine ASCs were refractory to activin A, bone morphogenetic protein-4 and Dickkopf-1-induced upregulation of cardiomyogenic marker expression, characteristic for in vitro cardiomyogenesis. Follow-up studies are required to further investigate the effect of isolation methods and tissue sources on clinically relevant multilineage differentiation potential in equine MSCs. Moreover, the use of stem cells with higher pluripotency should be considered for in vitro cardiomyogenesis in further studies.
Authors: Sung-Bae Kim, Tadaomi Furuta, Genta Kamiya, Nobuo Kitada, Ramasamy Paulmurugan, Shojiro A. Maki
Journal: Sensors
Imaging protein–protein interactions (PPIs) is a hot topic in molecular medicine in the postgenomic sequencing era. In the present study, we report bright and highly sensitive single-chain molecular strain probe templates which embed full-length Renilla luciferase 8.6-535SG (RLuc86SG) or Artificial luciferase 49 (ALuc49) as reporters. These reporters were deployed between FKBP-rapamycin binding domain (FRB) and FK506-binding protein (FKBP) as a PPI model. This unique molecular design was conceptualized to exploit molecular strains of the sandwiched reporters appended by rapamycin-triggered intramolecular PPIs. The ligand-sensing properties of the templates were maximized by interface truncations and substrate modulation. The highest fold intensities, 9.4 and 16.6, of the templates were accomplished with RLuc86SG and ALuc49, respectively. The spectra of the templates, according to substrates, revealed that the colors are tunable to blue, green, and yellow. The putative substrate-binding chemistry and the working mechanisms of the probes were computationally modeled in the presence or absence of rapamycin. Considering that the molecular strain probe templates are applicable to other PPI models, the present approach would broaden the scope of the bioassay toolbox, which harnesses the privilege of luciferase reporters and the unique concept of the molecular strain probes into bioassays and molecular imaging.