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Bio Reagents & Consumables
pEASY Cloning Vectors
pEASY® -Blunt3 Cloning Kit
pEASY®- Blunt3 Cloning Vector provides dual EcoR I and dual Not I enzyme digestion sites.
Brand: TransGen Biotech
Not Available
Free delivery within Singapore only
pEASY®- Blunt3 Cloning Vector provides dual EcoR I and dual Not I enzyme digestion sites. It is designed for cloning and sequencing Pfu-amplified PCR products. The cloned insert can be released from a single enzyme digestion.
•5 minutes fast ligation of Pfu-amplified PCR products.
•Ampicillin resistance gene for selection.
•Easy blue/white selection.
•T7 promoter, SP6 promoter, M13 forward and M13 reverse primers for sequencing.
•T7 promoter and SP6 promoter for in vitro transcription.
•Trans1-T1 Phage Resistant Chemically Competent Cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.
Storage
Competent Cell at -70°C for six months; others at -20°C for 9 months
Shipping
Dry ice (-70°C)
Product Contents
| Component | CB301-01 (20 rxns) | CB301-02 (60 rxns) |
| pEASY ®-Blunt3 Cloning Vector (10 ng/μl) | 20 μl | 3×20 μl |
| Control Template (5 ng/μl) | 5 μl | 5 μl |
| Control Primers (10 μM) | 5 μl | 5 μl |
| M13 Forward Primer (10 μM) | 50 μl | 150 μl |
| M13 Reverse Primer (10 μM) | 50 μl | 150 μl |
| Trans1-T1 Phage Resistant Chemically Competent Cells | 10×100 μl | 30×100 μl |
Specification :
| Product Contents | Kindly refer to the "Overview" tab. |
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