SingaporeSG
ChinaCN
MalaysiaMY
IndonesiaID
MyanmarMM_pddqnqv8.png)
Bio Reagents & Consumables
pEASY Cloning Vectors
pEASY® -T5 Zero Cloning Kit
pEASY®-T5 Zero Cloning Vector contains a suicide gene. Ligation of PCR fragment disrupts the expression of the gene. Cells that contain non-recombinant vector are killed upon plating. Therefore, blue/white screening is not required.
•5 minutes fast ligation of Taq-amplified PCR products.
•High cloning efficiency. Positive clones up to 100%.
•No blue/white selection needed.
•Suitable for short and large fragment cloning.
•Kanamycin and Ampicillin resistance genes for selection.
•M13 forward primer and M13 reverse primer for sequencing.
•T3 promoter and T7 promoter for in vitro transcription.
•Trans1-T1 Phage Resistant Chemically Competent Cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.
Storage
Competent Cell at -70°C for six months; others at -20°C for 9 months
Shipping
Dry ice (-70°C)
Product Contents
| Component | CT501-01 (20 rxns) | CT501-02 (60 rxns) |
| pEASY ®-T5 Zero Cloning Vector (10 ng/μl) | 20 μl | 3×20 μl |
| Control Template (5 ng/μl) | 5 μl | 5 μl |
| Control Primers (10 μM) | 5 μl | 5 μl |
| M13 Forward Primer (10 μM) | 50 μl | 150 μl |
| M13 Reverse Primer (10 μM) | 50 μl | 150 μl |
| Trans1-T1 Phage Resistant Chemically Competent Cells | 10×100 μl | 30×100 μl |
| Product Contents | Kindly refer to the "Overview" tab. |
No publications found...