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Bio Reagents & Consumables
Saccharomyces Cerevisiae
Yeast Nitrogen Base Media
Overview
Yeast Nitrogen Base media have been prepared according to the formulae of Wickerham (1943-51) and Burkholder (1943). Their research on the nutritional requirements of yeast strains resulted in the formulation of Yeast Nitrogen Base (YNB). YNB is a well-defined composition of salts, vitamins, amino acids and a nitrogen source for a vigorous growth of Sacharomyces Cerevisiae.
Besides the original formulation of YNB, Formedium™ produces many variations of this medium without one or more components. These YNB without ‘X’ media provide the researcher the possibility to grow yeast cells in the absence of a certain component or to replace a particular component.
Technical Data
|
Yeast Morphology Agar |
Yeast Nitrogen Base |
Yeast Potassium Nitrate Nitrogen Base |
Yeast Nitrogen Base w/o Amino Acids |
Yeast Nitrogen w/o Amino Acids and w/o Ammonium Sulfate |
Yeast Carbon Base |
Vitamin Free Yeast Base |
|
|
Nitrogen Source g/l |
|
|
|
|
|
|
|
|
Ammonium Sulphate |
3.5 |
5 |
– |
5 |
– |
– |
5 |
|
Asparagine |
1.5 |
– |
– |
– |
– |
– |
– |
|
Potassium Nitrate |
– |
– |
0.78 |
– |
– |
– |
– |
|
Caseine Hydrolysate |
– |
– |
– |
– |
– |
– |
– |
|
Carbon Source g/l |
|
|
|
|
|
|
|
|
Glucose.H2O |
10 |
– |
– |
– |
– |
10 |
10 |
|
Galactose |
– |
– |
– |
– |
– |
– |
– |
|
Raffinose |
– |
– |
– |
– |
– |
– |
– |
|
Amino Acids mg/l |
|
|
|
|
|
|
|
|
Histidine.HCl |
10 |
10 |
1 |
– |
– |
1 |
10 |
|
Methionine |
20 |
20 |
2 |
– |
– |
2 |
20 |
|
Tryptophan |
20 |
20 |
2 |
– |
– |
2 |
20 |
|
Vitamins µg/l |
|
|
|
|
|
|
|
|
Biotin |
2 |
2 |
2 |
2 |
2 |
2 |
– |
|
Ca-Panthotenate |
400 |
400 |
400 |
400 |
400 |
400 |
– |
|
Folic Acid |
2 |
2 |
2 |
2 |
2 |
2 |
– |
|
Inositol |
2000 |
2000 |
2000 |
2000 |
2000 |
2000 |
– |
|
Nicotinic Acid |
400 |
400 |
400 |
400 |
400 |
400 |
– |
|
p-Aminobenzoic Acid |
200 |
200 |
200 |
200 |
200 |
200 |
– |
|
Pyridoxine HCl |
400 |
400 |
400 |
400 |
400 |
400 |
– |
|
Riboflavin |
200 |
200 |
200 |
200 |
200 |
200 |
– |
|
Thiamine HCl |
400 |
400 |
400 |
400 |
400 |
400 |
– |
|
Trace Elements µg/l |
|
|
|
|
|
|
|
|
Boric Acid |
500 |
500 |
500 |
500 |
500 |
500 |
500 |
|
Copper Sulfate |
40 |
40 |
40 |
40 |
40 |
40 |
40 |
|
Potsssium Iodide |
100 |
100 |
100 |
100 |
100 |
100 |
100 |
|
Ferric Chloride |
200 |
200 |
200 |
200 |
200 |
200 |
200 |
|
Manganese Sulfate |
400 |
400 |
400 |
400 |
400 |
400 |
400 |
|
Sodium Molybdate |
200 |
200 |
200 |
200 |
200 |
200 |
200 |
|
Zinc Sulfate |
400 |
400 |
400 |
400 |
400 |
400 |
400 |
|
Minerals g/l |
|
|
|
|
|
|
|
|
KH2PO4 |
1 |
1 |
1 |
1 |
1 |
1 |
1 |
|
Magnesium Sulphate.anh |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
0.5 |
|
Sodium Chloride |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
|
Calcium Chloride.anh |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
0.1 |
|
Agar g/l |
|
|
|
|
|
|
|
|
Agar |
18 |
– |
– |
– |
– |
– |
– |
Product Range
- Yeast Nitrogen Base without Amino Acids
- Yeast Nitrogen Base without Amino Acids and without Ammonium Sulphate
- Yeast Carbon Base
- Yeast Morphology Agar
- Yeast Nitrogen Base
- Yeast Potassium Nitrate Nitrogen Base
- Yeast Vitamin Free Base
For more details, kindly refer to the "Downloads" tab.
| Product Range | Kindly refer to the "Downloads" tab. |
