TransScript® Uni Reverse Transcriptase [M-MLV, RNase H-](High Temperature RT)

TransScript® Uni Reverse Transcriptase is a reverse transcriptase with high thermal stability expressed and purified from E.  coli after genetic modification and screening. It enables a broard range of reaction temperature (42°C-65°C) with ultra-high  thermal stability and deficient RNase H activity. Its high reaction temperature is beneficial to open RNA secondary structure  when synthesizing the first-strand cDNA, with the optimal reaction temperature of 50°C. It also has the characteristics of high  sensitivity, high specificity, high yield (more full-length cDNA), High thermal stabilityand long half-life.

Features 

· Ultra-high thermal stability: reaction temperature 42°C-65°C. 

· High sensitivity, high specificity, high highyield and high processivity. 

· The unique design of Anchored Oligo (dT)₂₀ can anchor the first base next to the mRNA Poly (A)+5’end, and the binding  site is anchored, with high specificity, ensuring the efficiency and success rate of first-strand cDNA synthesis. 

· Deficient RNase H activity. The degradation of the template RNA in the DNA/RNA hybrid in the first-strand cDNA synthesis  reaction is avoided, thereby ensuring the yieldand length of the first-strand cDNA. 

· Synthesis length can be up to 20kb. 

Applications

· High-copy number and low-copy number gene detection. 

· High GC or RNA template with complex secondary structure. 

· cDNA library construction, primer extension, 3’ and 5’ RACE.

Storage
at -20°C for two years

Shipping
Dry ice (-70°C)