Unique genomic DNA remover is combined with TransScript® II First- Strand cDNA Synthesis SuperMix to achieve simultaneous genomic DNA removal and cDNA synthesis at 42-55°C. After cDNA synthesis, gDNA remover and TransScript® II RT/RI Enzyme Mix are inactivated by heating at 85°C for 5 seconds.
• Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination.
• The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR.
• After reaction, heat inactivation for RT/RI and gDNA remover simultaneously can avoid damage to RNA caused by heat inactivating DNase I used for pretreatment in traditional protocol.
• Simple and fast procedure.
• cDNA up to 15 kb.
Applications
• cDNA library construction, 3’ and 5’ RACE
• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template
Storage
at -20°C for two years
Shipping
Dry ice (-70°C)
Product Contents
| Component |
AH311-02 (50 rxns) |
AH311-03 (100 rxns) |
| TransScript® II RT/RI Enzyme Mix |
50 μl |
100 μl |
| gDNA Remover |
50 μl |
100 μl |
| 2×TS II Reaction Mix |
500 μl |
1 ml |
| Random Primer (0.1 μg/μl) |
50 μl |
100 μl |
| Anchored Oligo(dT)20 Primer (0.5 μg/μl) |
50 μl |
100 μl |
| RNase-free Water |
500 μl |
1 ml |
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