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Life Science
Tissue Grinder and Dissociator
TIGR Tissue Grinder & Dissociator
Modern cell research is increasingly relying on single cell analysis. Whether it is cell analysis in microbiology, immune-oncology or drug discovery processes, gentle single cell dissociation from their matrix is a prerequisite for proper single cell analysis.
OLS, your ideal cell research partner, is proud to present to you TIGR Tissue Grinder and Dissociator, the semi-automated, enzyme-free, fast and reproducible approach of generating pure and individual single cells from tissue samples.
TIGR is compatible with standard cell culture consumables 50 ml centrifuge tubes and standard cell strainer for mechanically, non-enzymatic and parallel processing of tissue samples in a reduced processing time.
TIGR is composed of a benchtop instrument and the grinding tube, a sterile disposable with the integrated core unit and cell strainer. The two-part core unit, consisting of rotor and stator inserts is equipped with counter rotating grinding teeth.
One of the main advantages of TIGR is its flexibility. The process parameters time, direction of rotation, acceleration, speed and the number of process steps can be adjusted by the researcher. Indeed, this allows creating an-experiment specific protocol for every application.
Another major advantage is saving time. The dissociation by TIGR was completed in less than two minutes. This is substantially less when benchmarked against other enzyme based technologies, methods that take 60 minutes, especially when multiple samples are processed in parallel.
Highlights
- Enzyme-free, standardized approach enables sophisticated applications
- 5 to 15x faster dissociation
- Integrated workflow for minimal contamination
- Easy to use software and customizable protocols
- Patented technology (EU, US, CN)
- Gentle sample preparation
- Easy to use software
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TIGR is compatible with standard cell culture consumables 50 ml centrifuge tubes and standard cell strainer for mechanically, non-enzymatic and parallel processing of tissue samples in a reduced processing time.
TIGR is composed of a benchtop instrument and the grinding tube, a sterile disposable with the integrated core unit and cell strainer. The two-part core unit, consisting of rotor and stator inserts is equipped with counter rotating grinding teeth.
One of the main advantages of TIGR is its flexibility. The process parameters time, direction of rotation, acceleration, speed and the number of process steps can be adjusted by the researcher. Indeed, this allows creating an-experiment specific protocol for every application.
Another major advantage is saving time. The dissociation by TIGR was completed in less than two minutes. This is substantially less when benchmarked against other enzyme based technologies, methods that take 60 minutes, especially when multiple samples are processed in parallel.
Principle
Underlying principle of the TIGR Tissue Grinder and Dissociator
The grinding unit interlocks with rows of teeth that can be moved against each other. The free rotation of the grinding teeth draws the tissue sample into the free space applying adequate amounts of shearing and milling force to gently isolate single cell from the tissue. The space between the grinding teeth and their fin-shaped geometry allows efficient extraction of viable single cells. The stator is the part that does not move, while the rotor is moved bidirectionally, depending on your customizable programming, allowing for user-defined and -controlled dissociation of the cell tissue.
Established Protocols
| Using default protocol | With protocol optimization |
|---|---|
| Liver | Skin* |
| Lung | FFPE* |
| Heart | Brain |
| Kidney | Urinary bladder |
| Spleen | Thyroid |
| Stomach | Tonsil |
| Colon | Prostate |
| Pancreas | Endometrium |
| Lymph node | Ovary |
| Rectum |
The tissue types found in the left column already have established protocols provided alongside the TIGR Tissue Grinder & Dissociator, as well as a good database from multiple users in cell research. Because of this, any new user can rely on these protocols without needing to make major adjustments, unless for special requirements in the downstream application. The right column shows tissue types that have been proven to be compatible with the TIGR Tissue Grinder, but might need protocol optimization on a case-to-case basis. We are happy to assist in the establishment and testing of new protocols and provide data on these tissue types whereever available. *Skin and FFPE will benefit from the addition of dissociation enzymes during the grinding process.
Many more tissue types are currently in testing steps. If you have tissue not listed in this table, please let us know – we are happy to conduct tests and generate data in cooperation with end users.
Applications
- 3D Cell Culture
- Tissue Models – Spheroids, Organoids, Tumoroids
- Single Cell Counting
- Isolation of Primary Cells
- Cancer Cell Line Development
- Flow Cytometry
Workflow
The tissue grinding and dissociation with the TIGR follows a simple and standardized workflow. In sterile conditions, the tissue sample is placed between rotor and stator, alongside with some liquid for the cells to be suspended in. Then the cell strainer and the tube are placed over the grinding compartment. From here on out, the tube can be taken out of the sterile space. The tube has to be placed on a TIGR slot with the upside down, so the rotor can connect to the slot’s rotation gear. After using the TIGR software to execute the grinding protocol, the tube can be taken off the instrument and flipped right side up, so that the suspension will pass the cell strainer. The final step to receive a ready-to-use single-cell suspension is centrifugation and resuspension. This quick and easy-to-follow workflow is all it needs to reliably produce a suspension from your tissue sample, no enzymatic dissociation and deactivation necessary – this saves time, effort, money and potential points of error in your workflow.
| Established Protocols | Kindly refer to the "Overview" tab. |
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Despina Soteriou, Markéta Kubánková, Christine Schweitzer et. al. (2023)
Nat. Biomed. Eng.
uring surgery, rapid and accurate histopathological diagnosis is essential for clinical decision making. Yet the prevalent method of intra-operative consultation pathology is intensive in time, labour and costs, and requires the expertise of trained pathologists. Here we show that biopsy samples can be analysed within 30 min by sequentially assessing the physical phenotypes of singularized suspended cells dissociated from the tissues. The diagnostic method combines the enzyme-free mechanical dissociation of tissues, real-time deformability cytometry at rates of 100–1,000 cells s−1 and data analysis by unsupervised dimensionality reduction and logistic regression. Physical phenotype parameters extracted from brightfield images of single cells distinguished cell subpopulations in various tissues, enhancing or even substituting measurements of molecular markers. We used the method to quantify the degree of colon inflammation and to accurately discriminate healthy and tumorous tissue in biopsy samples of mouse and human colons. This fast and label-free approach may aid the intra-operative detection of pathological changes in solid biopsies.
Stefan Scheuermann, Jonas M. Lehmann, Ramkumar Ramani Mohan et. al. (2022)
Front. Med., Vol. 9
Recent advances hold promise of making personalized medicine a step closer to implementation in clinical settings. However, traditional sample preparation methods are not robust and reproducible. In this study, the TissueGrinder, a novel mechanical semi-automated benchtop device, which can isolate cells from tissue in a very fast and enzyme-free way is tested for cell isolation from surgically resected tumor tissues.
Despina Soteriou, Markéta Kubánková, Christine Schweitzer et. al. (2021)
medRxiv
Rapid and accurate histopathological diagnosis during surgery is critical for clinical decision-making. The prevalent method of intraoperative consultation pathology is time, labour and cost intensive and requires the expertise of trained pathologists. Here, we present an alternative technique for the rapid, label-free analysis of biopsy samples by sequentially assessing the physical phenotype of singularized, suspended cells in high-throughput. This new diagnostic pipeline combines enzyme-free, mechanical dissociation of tissues with real-time deformability cytometry at measurement rates of 100 – 1,000 cells/sec, and machine learning-based analysis. We show that physical phenotype parameters extracted from brightfield images of single cells can be used to distinguish subpopulations of cells in various tissues, without prior knowledge or the need for molecular markers. Further, we demonstrate the potential of our method for inflammatory bowel disease diagnostics. Using unsupervised dimensionality reduction and logistic regression, we accurately differentiate between healthy and tumorous tissue in both mouse and human biopsy samples. The method delivers results within 30 minutes, laying the groundwork for a fast and marker-free diagnostic pipeline to detect pathological changes in solid biopsies.