Saccharomyces Cerevisiae

Synthetic Complete Media

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Overview

Synthetic Complete Medium (SC medium) is based upon Yeast Nitrogen Base supplemented with a carbon source and in which each of the commonly encountered auxothropies is supplemented by a complete mixture of amino acids and vitamins. The amino acid, vitamin mixture contains all possible supplements, i.e. nothing is dropped out in contrast to “Drop Out” mixtures. Cultures on SC Media combined with cultures on Yeast Nitrogen Base without Amino Acids in combination with drop out mixtures can be used to select for auxothrophies as in Yeast Genetics.

Formedium™ provides SC media supplemented with a carbon source like glucose, galactose, raffinose, succinate or a combination of carbon sources. The SC media are available with or without Agar premixed.

SC Media amino acid and vitamin supplement is available as a separate mixture as well for addition to other types of yeast media to supplement auxothrophies.

Technical Data

	SC Agar / Glucose	SC Broth / Glucose	SC Agar / Galactose	SC Broth / Galactose	SC Agar / Raffinose	SC Broth / Raffinose	SC Agar / Succinate	SC Broth / Succinate	SC Agar / Galactose and Raffinose	SC Broth / Galactose and Raffinose	SC-amino acids supplement
Nitrogen Source g/l
Ammonium Sulphate	5	5	5	5	5	5	5	5	5	5	–
Carbon Source g/l
Glucose.H2O	20	20	–	–	–	–	–	–	–	–	–
Galactose	–	–	20	20	–	–	–	–	20	20	–
Raffinose	–	–	–	–	20	20	–	–	10	10	–
Succinate	–	–	–	–	–	–	33	33	–	–	–
Vitamins µg/l
Biotin	2	2	2	2	2	2	2	2	2	2	–
Ca-Panthotenate	400	400	400	400	400	400	400	400	400	400	–
Folic Acid	2	2	2	2	2	2	2	2	2	2	–
Inositol	2000	2000	2000	2000	2000	2000	2000	2000	2000	2000	–
Nicotinic Acid	400	400	400	400	400	400	400	400	400	400	–
p-Aminobenzoic Acid	200	200	200	200	200	200	200	200	200	200	–
Pyridoxine HCl	400	400	400	400	400	400	400	400	400	400	–
Riboflavin	200	200	200	200	200	200	200	200	200	200	–
Thiamine HCl	400	400	400	400	400	400	400	400	400	400	–
Trace Elements µg/l
Boric Acid	500	500	500	500	500	500	500	500	500	500	–
Copper Sulfate	40	40	40	40	40	40	40	40	40	40	–
Potsssium Iodide	100	100	100	100	100	100	100	100	100	100	–
Ferric Chloride	200	200	200	200	200	200	200	200	200	200	–
Manganese Sulfate	400	400	400	400	400	400	400	400	400	400	–
Sodium Molybdate	200	200	200	200	200	200	200	200	200	200	–
Zinc Sulfate	400	400	400	400	400	400	400	400	400	400	–
Minerals g/l
KH2PO4	1	1	1	1	1	1	1	1	1	1	–
Magnesium Sulphate.anh	0.5	0.5	0.5	0.5	0.5	0.5	0.5	0.5	0.5	0.5	–
Sodium Chloride	0.1	0.1	0.1	0.1	0.1	0.1	0.1	0.1	0.1	0.1	–
Calcium Chloride.anh	0.1	0.1	0.1	0.1	0.1	0.1	0.1	0.1	0.1	0.1	–
Agar g/l
Agar	18	–	18	–	18	–	18	–	18	–	–
Supplements mg/l
Adenine Sulphate	18	18	18	18	18	18	18	18	18	18	18
Alanine	76	76	76	76	76	76	76	76	76	76	76
Arginine	76	76	76	76	76	76	76	76	76	76	76
Asparagine	76	76	76	76	76	76	76	76	76	76	76
Aspartic Acid	76	76	76	76	76	76	76	76	76	76	76
Cysteine	76	76	76	76	76	76	76	76	76	76	76
Glutamine	76	76	76	76	76	76	76	76	76	76	76
Glutamic Acid	76	76	76	76	76	76	76	76	76	76	76
Glycine	76	76	76	76	76	76	76	76	76	76	76
Histidine	76	76	76	76	76	76	76	76	76	76	76
Inositol	76	76	76	76	76	76	76	76	76	76	76
Isoleucine	76	76	76	76	76	76	76	76	76	76	76
Leucine	360	360	360	360	360	360	360	360	360	360	360
Lysine	76	76	76	76	76	76	76	76	76	76	76
Methionine	76	76	76	76	76	76	76	76	76	76	76
PABA	8	8	8	8	8	8	8	8	8	8	8
Phenylalanine	76	76	76	76	76	76	76	76	76	76	76
Proline	76	76	76	76	76	76	76	76	76	76	76
Serine	76	76	76	76	76	76	76	76	76	76	76
Threonine	76	76	76	76	76	76	76	76	76	76	76
Tryptophan	76	76	76	76	76	76	76	76	76	76	76
Tyrosine	76	76	76	76	76	76	76	76	76	76	76
Uracil	76	76	76	76	76	76	76	76	76	76	76
Valine	76	76	76	76	76	76	76	76	76	76	76

Product Range

SC Agar / 2% Glucose
SC Broth / 2% Glucose
SC Agar / 2% Galactose
SC Broth / 2% Galactose
SC Agar / 2% Raffinose
SC Broth / 2% Raffinose
SC Agar / 3.3% Succinate
SC Broth / 3.3% Succinate
SC Agar / 2% Galactose / 1% Raffinose
SC Broth / 2% Galactose / 1% Raffinose

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Specification :

Product Range

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GSS-brochure-Saccharomyces_Cerevisiae5.pdf

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Bio Reagents & Consumables

Saccharomyces Cerevisiae

Synthetic Complete Media

Overview

Technical Data

Product Range

Videos coming soon...