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Bio Reagents & Consumables
Precast Gels
SurePAGE Precast Gel
Overview
GenScript's Bis-Tris precast gel series are high performance polyacrylamide gels that are designed to separate a wide range of protein sizes by electrophoresis. The gels are cast in a neutral pH buffer that minimizes polyacrylamide hydrolysis and increases gel stability. They also run at neutral pH which minimizes protein modification compared to Tris-glycine gels.
Proteins are separated on SurePAGE using MOPS or MES running buffer. We recommend using MOPS buffer for protein sizes that are >30kD and MES for proteins that are <30 kD. We recommend using 4×LDS sample buffer (M00676) as the sample loading buffer.
Why Choose GenScript Gels?
- Sharp and consistent bands
- Fast run times
- Cost effective
- Large well volume
- Room temperature storage
- Compatible cassette design
Choosing the right PAGE gel
1. Gradient or homogenous concentrations:
- Gradient options: 4 – 12%, 8 – 16%, 4 – 20%
- Homogenous options: 8%, 10%, 12%
2. Number of wells:
- 10-well, 12-well or 15-well
3. Brand or model of PAGE gel tanks you are using, and are compatible with our SurePAGE™ Gels:
i. Bio-Rad Mini-PROTEAN® II & 3*
ii. Bio-Rad Mini-PROTEAN®Tetra System*
iii. Invitrogen Novex XCell I, II, & Surelock® (Use with GenScript Gel Tank Adapter Plates)
iv. LONZA PAGEr® Minigel Chamber
v. Hoefer Mighty Small (SE 260/SE 250) Hoefer Tall Mighty Small (SE 280)
vi. Cleaver Scientific OmniPAGE Mini Vertical Protein Electrophoresis System
Specifications
| Gel Thickness | 1.0 mm |
| Gel Chemistry | Bis-Tris |
| Gel Format | Mini Gels (10 x 8 cm) |
| Storage and Shelf Life | 18 months at 2-8°C; Or 6 months at room temperature (25°C) within 1 year after manufacturing date |
| Running Buffer Recommendations | Tris-MOPS SDS (Cat. No. M00138) or MES SDS (Cat. No. M00677) |
SurePAGE™ 10x8 gels
Related product
| Specifications | Kindly refer to the "Overview" tab. |
