Spike DELTA mRNA

Applications

For the COVID-19 vaccines, scientists developed synthetic mRNA in a lab that instructs cells to produce the distinctive spike protein from the SARS-CoV-2 virus. The immune system then targets and destroys these foreign spike proteins. If the body encounters the real virus at a later time, the body’s immune system will already be prepared to fend it off again. This mRNA encodes for the DELTA mutant Spike protein of the SARS-CoV-2 virus and bears five mutations. 

Spike protein is used as antigen for immunization and biochemical studies. For Research Use Only. Not for use in humans. Not for use in diagnostic or therapeutic purposes. Spike mRNAs resemble fully matured mRNAs with 5’cap1 structure and 3’ polyA tail, therefore ready to be translated by the ribosome.

mRNA transfection provides several advantages over plasmid DNA (pDNA) delivery. It does not require nuclear uptake for being expressed since translation of mRNA occurs directly into cytoplasm. Indeed, nuclear delivery (transport through nuclear membrane) is one the principal barriers for transfecting slow or non-dividing cells and consequently, mRNA transfection is particularly attractive for such purpose.This approach presents also the advantage of being non-integrative which is particularly appealing for stem cells, regenerative medicine or vaccine fields. Contrary to pDNA, mRNA cannot lead to genetic insertion causing mutations. Moreover, the protein expression from the mRNA is promoter-independent and faster than with DNA. 

For transfection we recommend RmesFect™ (#RM21000) and RmesFect™ Stem (#RS31000).