SIH Defined Minimal Media

Overview

This media is based on well defined formulations of mineral salts, vitamins and amino acids.

Two commonly used synthetic defined minimal Dictyostelium Discoideum media are FM Media and SIH Media.

FM medium developed by Franke and Kessin is used for transformation of Dictyostelium Discoideum and genomic studies. This formulation supports the growth of most strains that are capable of growing on HL5.

SIH medium is a newly developed modification of FM with regards to its amino acid compostion. Aspartic acid is added. Tryptophane and Lysine concentrations are significantly increased, resulting in an increase of cell density up to 5 x 10^7.

Both FM and SIH media are based on mineral salts in micro and macro concentrations supplemented with several vitamins. Besides these components, there is a large group of amino acids present. To facilitate genetic studies ForMedium™offers complete formulations of FM and SIH as well as both media lacking various amino acids such as without Arginine, Glutamic Acid, Lysine and Methionine.

New to our range of synthetic media are FM and SIH without all amino acids. These two newly developed media, combined with a range of amino acid drop-out mixtures gives the option to make every synthetic drop-out medium required for genetic studies.

Improvement of a synthetic medium for Dictyostelium discoideum,
Sang-In Han, Karl Friehs and Erwin Flaschel,
Process Biochemistry, 39 (8), 925 – 930, 2004

Product Range

• SIH Medium
• SIH medium w/o NH4Cl
• SIH medium w/o Amino acids
• SIH medium w/o Amino acids and w/o Ammonium chloride
• SIH medium w/o Arginine and w/o Lysine
• SIH medium w/o Arginine and w/o Lysine
• SIH medium w/o Cysteine and w/o Methionine
• SIH medium w/o Glutamic acid and w/o Lysine
• SIH Amino acid drop-out mixture
• SIH drop-out mixture, minus Arginine, 8495 mg/l
• SIH drop-out mixture, minus Cysteine, 8895 mg/l
• SIH drop-out mixture, minus Glutamic acid, 8650 mg/l
• SIH drop-out mixture, minus Lysine, 7945 mg/l
• SIH drop-out mixture, minus Methionine, 8845 mg/l
• SIH drop-out mixture, minus Arginine and w/o Cysteine, 8195 mg/l
• SIH drop-out mixture, minus Arginine and w/o Glutamic acid, 7950 mg/l
• SIH drop-out mixture, minus Arginine and w/o Lysine, 7245 mg/l
• SIH drop-out mixture, minus Arginine and w/o Methionine, 8145 mg/l
• SIH drop-out mixture, minus Cysteine and w/o Glutamic acid, 8350 mg/l
• SIH drop-out mixture, minus Cysteine and w/o Lysine, 7645 mg/l
• SIH drop-out mixture, minus Cysteine and w/o Methionine, 8545 mg/l
• SIH drop-out mixture, minus Glutamic acid and w/o Lysine, 7400 mg/l
• SIH drop-out mixture, minus Glutamic acid and w/o Methionine, 8300 mg/l
• SIH drop-out mixture, minus Lysine and w/o Methionine, 7595 mg/l

For more details, kindly refer to the "Downloads" tab.