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PhenoCycler Screened and Community Based Antibodies

PhenoCycler Screened and Community Based Antibodies

PhenoCycler Screened and Community Based Antibodies

Akoya Biosciences PhenoCycler Screened and Community Based Antibodies
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Overview

The list containing antibodies that will work with PhenoCycler have been “Screened” and tested in the Akoya Biosciences lab internally or tested and worked by the “community”. This is as a reference to any uses who would like to capitalise on PhenoCycler’s Chemistry and would like to integrate their own antibodies to this workflow.

Please refer HERE if you would like to purchase the inventoried antibodies containing the conjugated barcode and their complementary fluorophore.

 

PhenoCycler Component Reference Map

 

Please note that you will need to purchase the following items:

 

Details

PhenoCycler Screened and Community Based Antibodies
Target Phenotype Tissue Tissue Type Category
CD47 (TSP-1) "Don't eat me" Human FFPE Tonsil, Prostate Screened
CD227 (Muc-1) epithelial cells Human FFPE Various, Meningioma Screened
Helios Transcription Factor Human FFPE Tonsil, Prostate Screened
CXCR2 Myeloid (Neutrophil) Human FFPE Tonsil, Prostate Screened
AMACR Tumor Human FFPE Tonsil, Prostate Screened
PSMA Tumor Human FFPE Tonsil, Prostate Screened
CD86 Myeloid Human FFPE Tonsil, Prostate Screened
CD74 Myeloid Human FFPE Tonsil, Prostate Screened
CD172 Myeloid Human FFPE Tonsil, Prostate Screened
Vimentin Mesenchymal cells Mouse FFPE Brain, Spleen, Tumor Screened
F4/80 macrophages Mouse FFPE Spleen, Tumor Screened
TIM3 T cells supression, macrophages, denditric cells Human FFPE Various Screened
CD206 M2 Macrophages Human FFPE Tonsil, Prostate, Tumor TMA Screened
CD1c Myeloid (Dendritic) Human FFPE Tonsil, Prostate Screened
LOX-1 Endothelial, Macrophages, Cardiomyocytes Human FFPE Tonsil, Prostate Screened
NKp46 NK cell Human FFPE Tonsil, Bladder Screened
NKG2A NK cell Human FFPE Tonsil, Bladder Screened
DNAM-1 NK cell Human FFPE Tonsil, Bladder Screened
CXCR3 NK cell Human FFPE Tonsil, Bladder Screened
CD56 NK cell Human FFPE, Mouse FFPE Tonsil, Spleen, Tumor Screened
NKG2D NK cell Human FFPE H&N, Ovarian Cancer Screened
CD16 NK cell Human FFPE Tonsil, Prostate Screened
CD16 NK cell Human FFPE H&N, Ovarian Cancer Screened
CD56 NK cell Human FFPE H&N, Ovarian Cancer Screened
CD163 Myeloid Human FFPE Various Screened
Keratin5 Basal keratinocytes/Basal tumor subtype Human FFPE Breast Screened
Keratin8 Luminal epithelial cells/Luminal tumor subtype Human FFPE Breast Screened
Keratin19 Luminal epithelial cells/Luminal tumor subtype Human FFPE Breast Screened
Col1A1 ECM organization Mouse FFPE Spleen, Tumor, Brain Screened
camKII Excitatory neurons Rat FF Brain Screened
CCR2 Monocytes/macrophages NHP FFPE lung Screened
CD206 Macrophages NHP FFPE lung Screened
HER2 Luminal breast cancer (Huang et al 2021) Human FFPE Breast Screened
CD28 T cells NHP FFPE lung Screened
CNP1 Dendritic cells, Langerhans cells Rat FF Brain Screened
ERAP1 Neurons Human FFPE Ovarian Screened
CD11b Myeloid cells Human FFPE Various Screened
MICA/B "Kill me" Human FFPE H&N, Ovarian, TMA Screened
P21 Negative regulator of cell cycle/cell cycle arrest Human FF Brain Organoids Screened
P62 Autophagy marker, defective protein clearance Human FF Brain Organoids Screened
STING Microglia, vasculature, immune cells, tumor cells, stimulator of interferon genes (Anti-tumor) Human FFPE Ovarian Screened
TfR Endothelial cells Human FFPE, NHP FFPE Brain Screened
MAP-2 Neurons Human FFPE, Mouse FFPE, NHP FFPE Brain Screened
H2A.X DNA damage Human FFPE, Mouse FFPE, NHP FFPE Brain Screened
GFAP Astrocytes Human FFPE, Mouse FFPE, NHP FFPE Brain Screened
PSD-95 Synaptic marker/neurons Human FFPE, Mouse FFPE, NHP FFPE Brain Screened
NeuN Neurons Human FFPE, Mouse FFPE, NHP FFPE Brain Screened
Synaptophysin Synapses Human FFPE, Mouse FFPE, NHP FFPE Brain Screened
Olig-2 Oligodendrocyte Human FFPE, Mouse FFPE, NHP FFPE Brain Screened
VGLUT1 Neurons Human FFPE, Mouse FFPE, NHP FFPE Brain Screened
Doublecortin Neurons Human FFPE, Mouse FFPE  Brain Screened
MBP Oligodendrocyte Human FFPE, Mouse FFPE, NHP FFPE Brain Screened
GAD67 Neurons Human FFPE, Mouse FFPE, NHP FFPE Brain Screened
Serotonin Tr Neurons Human FFPE, Mouse FFPE  Brain Screened
cTIP Neurons Human FFPE, Mouse FFPE  Brain Screened
Calbindin Neurons Human FFPE, Mouse FFPE  Brain Screened
LamB1 Nucleated cells Human FFPE, Mouse FFPE  Brain Screened
Neurofilament Neurons Human FFPE, Mouse FFPE, NHP FFPE Brain Screened
TMEM119 Microglia Human FFPE, Mouse FFPE  Brain Screened
AQP4 Astrocytes Human FFPE, Mouse FFPE  Brain Screened
A-beta Neurons, glia Human FFPE, Mouse FFPE  Brain Screened
Iba1 Macrophages/ Microglia Human FF Pancreas Community
CD31 Endothelial cells Mouse FFPE Brain Screened
P-Tau (S396) Neurons Mouse FFPE Brain Screened
CD68 Macrophages, monocytes Mouse FFPE Brain Screened
APOE Hepatocytes, macrophages, and neurons Human FFPE Brain Screened
Tau (pSer202) Neurons Human FFPE Brain Screened
Tau Neurons Human FFPE Brain Screened
Claudin-5 Vascular Human FFPE, NHP FFPE Brain Screened
ChAt Neurons Human FFPE, NHP FFPE Brain Screened
TH Neurons Human FFPE, NHP FFPE Brain Screened
Sox9 Chondrocytes, Sertoli Cells, Stem and Progenitor Cells, Neural Cells Human FFPE, NHP FFPE Brain Screened
GAD1 Neurons Human FFPE, NHP FFPE Brain Screened
VIAAT Neurons, GABAergic and glycinergic cells Human FFPE, NHP FFPE Brain Screened
LRP1 Neurons, hepatocytes, and immune cells Human FFPE, NHP FFPE Brain Screened
SOD1 Neurons, erythrocytes, and immune cells Human FFPE, NHP FFPE Brain Screened
IGF-IR Fibroblasts, muscle cells, and immune cells Human FFPE, NHP FFPE Brain Screened
NPR-C? Smooth muscle cells, fibroblasts NHP FFPE Brain Screened
LRP2? Kidney cells and Epithelial cells NHP FFPE Brain Screened
PTH1R? Bone cells, kidney cells NHP FFPE Brain Screened
SGLT2? Renal proximal tubule cells NHP FFPE Brain Screened
ASCT2 Neurons, tumor cells and immune cells Human FFPE Various Screened
IDH2 Tumor cells Human FFPE Various Screened
MC Tryptase Mast cells Human FFPE Various Screened
LC3B Tumor cells Human FFPE Various Screened
Cleaved PARP Apoptotic cells Human FFPE Various Screened
BCL-XL Lymphocytes and tumor cells Human FFPE Various Screened
NaKATPase Neurons, muscle cells and epithelial cells Human FFPE Various Screened
Cytochrome c Apoptotic cells Human FFPE Various Screened
CD227 Epithelial cells Human FFPE Various Screened
LEF1 T cells Human FFPE Various Screened
C1Qa Kupffer cells (macrophages), monocytes Human FFPE Various Screened
GLUT1 Erythrocytes and tumor cells Human FFPE Various Screened
Cyclin D1 Proliferating cells Human FFPE Various Screened
BAD Apoptotic cells Human FFPE Various Screened
BAX Apoptotic cells Human FFPE Various Screened
CD138 Plasma cells Human FFPE Various Screened
pNRF2 Cells undergoing oxidative stress Human FFPE Various Screened
CPT1A Cells involved in fatty acid metabolism Human FFPE Various Screened
CD2 T cells and NK cells Human FFPE Various Screened
CD1a Dendritic cells Human FFPE Various Screened
ATPA5 Metabolic: TCA cycle Human FFPE Various Screened
Citrate Synthase Metabolic: TCA cycle Human FFPE Various Screened
CK17 Epithelial cells Human FFPE Various Screened
CD7 T cells and NK cells Human FFPE Various Screened
G6PD Metabolic: Pentose Phosphate Pathway Human FFPE Various Screened
CD69 T cells and NK cells Human FFPE Various Screened
HLA-DPB1 B cells, dendritic cells and macrophages Human FFPE Various Screened
Pax5 B cells Human FFPE Various Screened
CCR6 B cells Human FFPE Various Screened
AXL Signaling: Immune evasion Human FFPE Various Screened
CD15 Granulocytes Human FFPE Various Screened
CK19 Epithelial cells Human FFPE Various Screened
CD163 M2 Macrophages Human FFPE Various Screened
SDHA Metabolic: TCA cycle Human FFPE Various Screened
LaminB1 Ubiquitous: Nuclear lamen Human FFPE Various Screened
ZEB1 Tumor cells: epithelial-mesenchymal transition  Human FFPE Various Screened
HK1 Metabolic: Glycolysis Human FFPE Various Screened
LDHA Metabolic: Glycolysis Human FFPE Various Screened
MMP-9 Metabolic: Invasion Human FFPE Various Screened
pRPS6 Signaling: Proliferation Human FFPE Various Screened
Galectin 9 Signaling: Apoptosis Human FFPE Various Screened
CD57 NK cells and cytotoxic T cells Human FFPE Various Screened
Beclin-1 Stress: Autophagy Human FFPE Various Screened
ZAP70 T cells and NK cells Human FFPE Various Screened
NOX2 Phagocytes Human FFPE GBM Screened
CD172 (SIRPα) Myeloid cells, stem cells, neurons Human FFPE GBM Screened
CD161 NK cells and some lymphocytes Human FFPE lymph node; nasopharyngeal carcinoma  Screened
p21 Senescence Human FFPE  nasopharyngeal carcinoma  Screened
Glucagon pancreatic alpha cells Mouse FFPE Xenograft, Human FFPE Kidney Screened
Insulin pancreatic beta cells Mouse FFPE Xenograft, Human FFPE Kidney Screened
Cardiac Troponin Myocardial injury (cardiomyyocytes) Human FFPE Heart Screened
Integrin beta 1 (CD29) Leukocytes, adipose-derived stem cells and BM-MSCs Human FFPE Kidney Screened
PR PR positive tumor cells Human FFPE Breast Screened
FAP Fibroblasts Human FFPE Breast, TMA Screened
Transgelin smooth muscle cells Human FFPE TMA Tumor Screened
Ficolin  Monocytes,neutrophils Human FFPE TMA Tumor Screened
CD54 Endothelial cells, activated lymphocytes Human FFPE TMA Tumor Screened
CD27 B and T cell activation Human FFPE TMA Tumor Screened
CD20 B cells Mouse FFPE Spleen, Tumor Screened
CD8 Cytotoxic T cells Mouse FFPE Spleen, Tumor Screened
CD11c Dendritic cells Mouse FFPE Spleen, Tumor Screened
CD31 endothelal cells Mouse FFPE Spleen, Tumor Screened
CD44 Cancer Stem Cells Mouse FFPE Spleen, Tumor Screened
Foxp3 T cells (T reg) Mouse FFPE Spleen, Tumor Screened
CD3e Mature T cells Mouse FFPE Spleen, Tumor Screened
CD36 Human Adipocyte Progenitors Mouse FFPE Spleen, Tumor Screened
CD98hc (SLC3A2) Endothelial cells, neuropil Human FFPE, NHP FFPE Brain Screened
CD206 Macrophages (M2) Mouse FFPE Spleen, Tumor Screened
FcRγ Myeloid cells, Nk cells, Macrophages Mouse FFPE Spleen, Tumor Screened
CGRP sensory nerves; also expressed by  fibroblasts, keratinocytes,T lymphocytes, B lymphocytes, and monocytes Mouse FF Bone Marrow Screened
LePR perivascular cells Mouse FF Bone Marrow Screened
Iba1 Glia Mouse FFPE Spleen, Tumor Screened
Flk2 hematopoietic stem cell differentiation Mouse FF Bone Marrow Screened
CD68 Macrophages, monocytes Mouse FFPE Spleen, Tumor Screened
CD45 nucleated Hematopoietic cells (Pan Immune) Mouse FFPE Spleen, Tumor Screened
S100A9 Neutrophils, macrophages Mouse FFPE Spleen, Tumor Screened
Ly6g Peripheral neutrophils, monocytes, granulocytes Mouse FFPE Spleen, Tumor Screened
MPO neutrophils NHP FFPE Spleen, Retina, Lung Screened
Calprotectin neutrophils NHP FFPE Spleen, Retina, Lung Screened
CD3e T cells NHP FFPE Spleen, Retina, Lung Screened
CD8a cytotoxic T cells NHP FFPE Spleen, Retina, Lung Screened
SLAM (CD150) peripheral blood hematopoietic stem and progenitor cells Mouse FF Bone Marrow Screened
Nkp46 NK cells Mouse FF Bone Marrow Screened
Fcgr(CD32/CD16) NK cells Mouse FF Bone Marrow Screened
Perlipin adipocytes Mouse FF Bone Marrow Screened
F4/80 macrophages Mouse FF Bone Marrow Screened
CD41 platelets and megakaryocytes Mouse FF Bone Marrow Screened
CD115 monocytes, macrophages Mouse FF Bone Marrow Screened
Endomucin endothelium Mouse FF Bone Marrow Screened
Tyrosine Hydroxylase dopaminergic neurons Mouse FF Bone Marrow Screened
CD105 angiogenic endothelial cells Mouse FF Bone Marrow Screened
CD19 B cells Human FFPE Various Screened
CD48 lymphocytes, monocytes Mouse FF Bone Marrow Screened
CD15 myeloid cells Human FFPE Various Screened
CD16 NK cells Human FFPE Various Screened
B7H3/CD276 Fibroblasts Human FFPE Human FFPE Head and Neck, Ovarian, Multitumor Cancer TMA Screened
CD227 epithelial cells Human FFPE Breast Organoids Screened
IRF4/MUM1 B cells, plasma cells Human FFPE Tonsil, Multi-tissue TMA Screened
CD278 activated CD4+ and CD8+ T cells  Human FFPE Tonsil Screened
CD30 activated T cells, NK cells, B cells Human FFPE Tonsil Screened
CD5 T cells Human FFPE Tonsil Screened
ER nuclear and membrane receptor localized in a subset mature luminal cells and luminal progenitor cells (Rosenbluth et al 2020) Human FFPE Breast Organoids Screened
GATA3 Transcription factor expressed in the luminal A subtype (Voduc et al 2008) Human FFPE Breast Organoids Screened
p53 Tumor supressor and transcription factor, expressed at low levels in unstressed cells (Aubrey et al 2017; Zhang et al 2018) Human FFPE GBM Screened
CD25 (IL2RA) T cells Human FFPE Various Screened
LMP1 Memory B cells in tonsil following Epstein-Barr virus infection (Babcock and Lawson 2000). Germinal center centroblasts and centrocytes (Babcock et al 2000) Human FFPE Tonsil Screened
EBNA1  Memory B cells in tonsil following Epstein-Barr virus infection (Babcock and Lawson 2000). Germinal center centroblasts and centrocytes (Babcock et al 2000) Human FFPE Tonsil Screened
EBNA2  Naive B cells (Babcock et al 2000) Human FFPE Tonsil Screened
LMP2A  EBV infected B cells (Rovedo and Longnecker 2007) Human FFPE Tonsil Screened
CD30  B lymphoblasts (Steiniger et al 2020) Human FFPE Tonsil Screened
EBNA2 Naive B cells (Babcock et al 2000) Human FFPE Tonsil Screened
LMP1 Memory B cells in tonsil following Epstein-Barr virus infection (Babcock and Lawson 2000). Germinal center centroblasts and centrocytes (Babcock et al 2000) Human FFPE Tonsil Screened
EBVEaD EBV infected B cells (Thermofisher) Human FFPE Tonsil Screened
S100A4 Fibroblasts and immune cells Human FFPE Various Screened
Tim-3 CD4 helper T cells and CD8 T lymphocytes (Wolf et al 2019) Human FFPE Tonsil Screened
NFkB p65 Infllammatory marker, Activated B cells  Human FFPE Tonsil Screened
ERG pan endothelial marker, lymphovascular invasion (Kim et al 2013) Human FFPE Breast Screened
Active YAP Epigenetics Human FFPE Tonsil Screened
BID Epigenetics Human FFPE Tonsil Screened
H3Me3K9 Epigenetics Human FFPE Tonsil Screened
H3Me3K27 Epigenetics Human FFPE Tonsil Screened
H4MeK20 Epigenetics Human FFPE Tonsil Screened
H3Me3K36 Epigenetics Human FFPE Tonsil Screened
H3AcK9 Epigenetics Human FFPE Tonsil Screened
H3Me123K79 Epigenetics Human FFPE Tonsil Screened
HDAC2 Epigenetics Human FFPE Tonsil Screened
Dnmt1 Epigenetics Human FFPE Tonsil Screened
HP1 alpha Epigenetics Human FFPE Tonsil Screened
Brd4 Epigenetics Human FFPE Tonsil Screened
pS139 gamma histone H2AX Epigenetics, double stranded DNA breaks Human FFPE TMA Tumor Screened
pY1068/Y1173 EGFR EGFR signaling Human FFPE TMA Tumor Screened
CA9 pH regulation/ Hypoxia Human FFPE TMA Tumor Screened
pT202 ERK1 + Y204 ERK2  ERK1/ ERK2 signaling Human FFPE TMA Tumor Screened
TGF beta TGF beta signaling Human FFPE TMA Tumor Screened
pT202 ERK1 + T185 ERK2 ERK1/ ERK2 signaling Human FFPE TMA Tumor Screened
CD27 Lymphocyte co-stimulation Mouse FF Bone Marrow Community
CD34 hematopoetic stem cells, hematopoetic progenitors, vascular endothelial cells Mouse FF Bone Marrow Community
CD47 Phagocytosis regulation, "don't eat me" Mouse FF Bone Marrow Community
CD62L T cell memory Mouse FF Bone Marrow Community
CD117 hematopoetic stem cells, hematopoetic progenitors Mouse FF Bone Marrow Community
CTLA4 Negative T cell costimulation Mouse FF Bone Marrow Community
CD144/VE-Cadherin Endothelial cells Mouse FF Bone Marrow Community
CD206 M2 Macrophage Mouse FF Bone Marrow Community
TCR Gamma/Delta Gamma Delta T cells, peptide and lipid antigen recognition Mouse FF Bone Marrow Community
Arginase 1 MDSCs/ Alternative activated Macrophages  Human FF Pancreas Community
C-peptide Beta cells of pancreas, Insulin secretion Human FF Pancreas Community
CD36 Atheroslcerosis Human FF Pancreas Community
CD86 Lymphocyte acitvation/ immune checkpoint marker Human FF Pancreas Community
CD163 M2 macrophage Human FF Pancreas Community
CD206 M2 Macrophage Human FF Pancreas Community
Chromagranin A Neuroendocrine cells Human FF Pancreas Community
Ghrelin Endocrine cells of GI tract Human FF Pancreas Community
Glucagon alpha cells of pancreatic islets of Langerhans Human FF Pancreas Community
Pancreatic Polypeptide/PP PP cells of Pancreas  Human FF Pancreas Community
Somatostatin Delta cells of Pancreas Human FF Pancreas Community
CD10 B lymphoblast cell development and leukemia Human FFPE Tonsil Community
CD123 Interleukin-3 receptor, plasmacytoid dendritic cells, various types of lukemia Human FFPE Tonsil Community
CD123 Interleukin-3 receptor, plasmacytoid dendritic cells, various types of lukemia Human FFPE Tonsil Community
CLEC9A Dendritic cell subsets Human FFPE Tonsil Community
DR5 TRAIL activated cell death signaling receptor Human FFPE Tonsil Community
NSE Neurons and  peripheral neuroendocrine cells Human FFPE Tonsil Community
CD93 Plasma cell/B cell Human FFE Meningioma Screened
IGFBP7 Insulin like growth factor binding protein Human FFE Meningioma Screened
PLA2G10 Phospholipase A2 Human FFE Meningioma Screened
CTLA4 Activated T cell Human FFPE Various Screened
SOX10 Transcription factor/Marker for malignant melanoma Human FFE Melanoma Screened
p21 Negative regulator of cell cycle/cell cycle arrest Human FFE Breast Screened
PTEN Tumor supressor  Human FFE Breast, Melanoma Screened
p120 Predictor of outcome in lobular neoplasia Human FFE Breast Screened
Bcl6 Transcription factor for regulator of TFH cells Human FFE Tonsil Screened

 

Specification :
Details Kindly refer to the "Overview" tab.
PhenoCycler Screened and Community Based Antibodies
PhenoCycler Screened and Community Based Antibodies
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