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Bio Reagents & Consumables
pEASY Prokaryotic Expression Vectors
pEASY®-Blunt E1 Expression Kit
pEASY®-Blunt E1 Expression Vector is constructed from pET vector, it utilizes a highly efficient, five-minute blunt cloning strategy to clone PCR product into high-efficient expression vector. The size of control insert is 750 bp, and expressed target protein is about 27 kDa.
• 5 minutes fast ligation of Pfu-amplified PCR products.
• Ampicillin resistance.
• T7 promoter primer and T7 terminator primer for sequencing.
• Bacteriophage T7lac promoter for high level expression.
• N-terminal 6×His tag for easy purification.
• Trans1-T1 phage resistant chemically competent cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.
• E1 Expression Plasmid included as negative control.
Storage
Trans1-T1 Phage Resistant Chemically Competent Cell at -70°C for six months; Others at -20°C for nine months.
Shipping
Dry ice (-70°C).
| Component | CE111-01 (10 rxns) |
| pEASY®-Blunt E1 Expression Vector (15 ng/μl) | 10 μl |
| E1 Expression Plasmid (Negative Control) (15 ng/μl) | 10 μl |
| EControl Template (5 ng/μl) | 10 μl |
| EControl Forward Primer (10 μM) | 10 μl |
| EControl Reverse Primer (10 μM) | 10 μl |
| T7 Promoter Primer (10 μM) | 50 μl |
| T7 Terminator Primer (10 μM) | 50 μl |
| Trans1-T1 Phage Resistant Chemically Competent Cell | 5×100 μl |
| Product Contents | Kindly refer to the "Overview" tab. |
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