OT-2
Putting laboratory automation within reach
Easy lab automation
The OT-2 benchtop pipetting robot is flexible and compatible and is suitable for a variety of common laboratory automation processes.
The most time-consuming processes in automated laboratories

NGS library preparation
NGS library preparation is one of the most common automated applications for the OT-2 automated pipetting platform. It automates not only NGS library preparation, but also other steps of the pre-sequencing workflow, including reagent dispensing, bead cleanup, sample concentration normalization, and pooling. You can use your own protocol, or choose from our online protocol library a protocol that has been validated by the Opentrons scientific team in similar applications or a protocol provided by a major reagent brand currently on the market.
PCR settings
Another commonly used automation application is PCR experiments, especially when you need to prepare large amounts of samples. You can use the OT-2 automated pipetting platform to mix samples and PCR mixes in reaction plates and then place the plates into a thermal cycler module or PCR instrument.
protein purification
The OT-2 automated pipetting platform provides a cost-effective solution for automated small-scale protein purification. The OT-2 automated pipetting platform can automate protein purification, proteomics sample processing, and enzymatic and chemical cleavage. OT-2 can currently implement two automated methods, namely dual-flow chromatography-based protein purification and magnetic bead-based protein purification. purification.
Nucleic acid extraction and purification
OT-2 provides a cost-effective solution that can easily implement tedious nucleic acid purification steps. If you are using SPRI (Solid Phase Reversibly Immobilized) magnetic beads for your separation and/or purification steps, you can automate these workflows using the OT-2 automated pipetting platform.
Routine reagent packaging
The OT-2 provides you with a quick and easy solution for handling common liquids in the laboratory. You can use the OT-2 automated pipetting platform to easily perform a range of basic liquid handling tasks, including centrifuge tube and microplate filling, serial dilution, precision selection, sample concentration homogenization and sample pooling. You can choose from a range of proven protocols in the online protocol library, create your own, or contact us to have our application engineering team customize a protocol for you.
Compact model, powerful function
Only half the size of a standard laboratory bench
- Metric (width, depth, height) 63 mammoth 57 mammoth 66 smart
- Imperial (width, depth, height) 25 in small 22.5 in small 26 in
Performance comparable to similar devices costing 10 times more
- Accuracy 15% at 1 μL
- Accuracy 5% at 1 μL
Start your laboratory automation
We provide you with the professional technical support you need for laboratory automation
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Online demo
Provide OT-2 online demonstration to answer your application questions
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Order OT-2
Select OT-2, pipettes, related modules and labware
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OT-2 Receiving and unpacking
We provide unpacking instructions to help you complete the unpacking process
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Customization and customer service support
On-site installation, development of customized protocols, etc.
How to find the protocol creation method that works best for you
A protocol is the code used to run the instrument through your experiment. We've established different ways to create protocols, so whether you're new to automation or an experienced Python programmer, there's something for you.