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    Opal Reagent Packs

    Opal Fluorophore Reagent Packs

    Opal Fluorophore Reagent Packs

    Opal™ is a practical workflow for simultaneous detection of up to 8 tissue biomarkers plus nuclear counterstain, within a single image.
    Pricing & Availability Download Catalogue Add to wishlist Akoya Biosciences
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    Overview

    Opal™ is a practical workflow for simultaneous detection of up to 8 tissue biomarkers plus nuclear counterstain, within a single image. The method is similar to standard immunohistochemistry and is accessible to many laboratories where standard IHC method development is performed. Standalone Opal Fluorophore reagent packs allow you to pick and choose your panel’s biomarker quantity and design.

     

    Catalog

    Catalog # Product Size
    FP1500001KT Opal 480 Reagent Pack Up to 50 Slides
    FP1487001KT Opal 520 Reagent Pack Up to 50 Slides
    FP1494001KT Opal 540 Reagent Pack Up to 50 Slides
    FP1488001KT Opal 570 Reagent Pack Up to 50 Slides
    FP1495001KT Opal 620 Reagent Pack Up to 50 Slides
    FP1496001KT Opal 650 Reagent Pack Up to 50 Slides
    FP1497001KT Opal 690 Reagent Pack Up to 50 Slides
    FP1501001KT Opal 780 Reagent Pack Up to 50 Slides
    FP1490 10X Spectral DAPI 1.5 mL

     

    Details

    Each Opal Reagent Pack contains 1 vial DMSO and enough Opal reagent for up to 50 slides in a multiplex IHC protocol.

    Opal 780 is a two-step antibody-mediated reaction and requires special considerations, please refer to the product technical data sheet.

     

    Workflow

    Bake and Deparaffinize Slides

    • Bake at 60 C for one hour
    • Xylene wash (3x ten minutes)
    • Rehydrate with EtOH gradient into di H2O

    Slide Fixation

    • 10% NBF for 20 minutes
    • Di H2O wash

    Antigen Retrieval

    • AR6 or AR9 microwave treatment (MWT)
    • Cool to RT for at least 15 minutes

    Blocking

    • Di H2O rinse, TBST rinse
    • PAP pen barrier
    • Incubate tissue for 10 minutes in blocking solution at RT

    Primary Antibody

    • Remove blocker and add primary antibody
    • Rinse with TBST, then wash 3 times for two minutes each in TBST

    Secondary Antibody

    • Incubation 10 minutes at RT
    • Rinse with TBST and wash 3 times for 2 minutes each

    Opal Reagent

    • Opal fluorophore incubation for ten minutes at RT
    • Rinse with TBST and wash 3 times for 2 minutes each

    MWT

    • Rinse slides with AR6 or AR9
    • Place slides in microwave-safe jar, perform MWT
    • Cool to RT for at least 15 minutes

    Spectral DAPI

    • Use AR6 for MWT prior to DAPI for removal of any unbound fluor
    • Rinse slides in di H2O and then TBST
    • Incubate DAPI for 5 minutes at RT
    • Wash with di H2O and TBST two minutes each

    Mount

    • Apply monunting medium for fluorescene microscopy for coverslip

     

    Related products

    PhenoImager™ HT

    Specification :
    Shipping Condition Blue Ice
    Sample Human and Mouse
    Product Category Opal Kits
    Type Opal Detection Kit
    Detection Method Instrument Compatibility Depends on Combination of Opal Reagents
    Welcome-to-Opal-Brochure-11-18-2022_Gaia_Science1.pdf
    Opal Fluorophore Reagent Packs
    Opal Fluorophore Reagent Packs
    Videos coming soon...

     
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