Opal 6-Plex Detection Kits - for Field of View Imaging (formerly Opal 7-Color IHC kits)

Overview

Opal™ 6-Plex Detection Kits for Field of View Imaging provide a practical workflow for the simultaneous detection of up to six tissue biomarkers plus nuclear counterstain within a single image. The method is similar to standard immunohistochemistry (IHC) and is accessible to many laboratories where standard IHC is performed.

Catalog

Details

The Opal 6-Plex Manual Detection Kit for Field of View Imaging contains:

• 6 reactive fluorophores (Opal 520, Opal 540, Opal 570, Opal 620, Opal 650 and Opal 690)
• DMSO
• 10X Spectral DAPI
• 1X Plus Manual Amplification Diluent
• 1X Antibody Diluent/Block
• 1X Opal Anti-Ms + Rb HRP
• 10X AR6 Buffer

The Opal 6-Plex Detection Kit for Field of View Imaging contains:

• 6 reactive fluorophores (Opal 520, Opal 540, Opal 570, Opal 620, Opal 650 and Opal 690)
• DMSO
• 10X Spectral DAPI
• 1X Plus Automation Amplification Diluent
• 1X Antibody Diluent/Block
• 1X Opal Anti-Ms + Rb HRP

Users should source primary antibodies and other workflow reagents separately. The Opal 6-Plex Detection Kits for Field of View Imaging were optimized for use on the Mantra 2, Vectra 3, and PhenoImager HT Systems. Kits contain enough reagents to stain up to 50-slides at the recommended 1:100 or 1:150 dilution ratio.

Opal provides researchers with the tools to interrogate multiple pathways while retaining context provided by tissue images. This approach provides information not available from alternative techniques like flow cytometry or analysis of single markers in serial sections.

Opal will enable you to:

• Measure up to six tissue biomarkers at once
• Use the best primary antibodies together in multiplex panel, with no species-based crosstalk
• Retain spatial cellular context that is lost in flow cytometry
• Confirm single cell co-expression for many biomarkers in one tissue section
• Get more information while conserving precious tissue.

Related products

PhenoImager™ HT