The Hitachi Chromaster 5610 mass detector is designed for HPLC applications, that may not require a large-scale mass spectrometer.
*:"Chromaster" is a registered trademark of Hitachi High-Tech Corporation in Germany and France.
Measurement result by scanning mode
The atmospheric ion filter can be removed in 3 steps, and it can be reused after ultrasonic cleaning.
Measurement result by scanning mode
The atmospheric ion filter can be removed in 3 steps, and it can be reused after ultrasonic cleaning.
Items | Description |
---|---|
Measurement mass range (m/z) | 20 - 1,000 |
Ion source | Electrospray ionization (ESI) |
Dimensions | 440(W) × 600(D) × 430(H) mm |
N2 gas usage | Max flow rate 3.4 L/min, Pressure 300 ± 20 kPa |
As the mobile phase is delivered by a pump, a small volume of sample is injected and measured by “Flow Injection Analysis Method”. By connecting the HPLC to an autosampler, the measurement can be automated and it can be used for the confirmation of mass information in each sample.
*1Flow injection analysis
Confirmation of the mass information for a compound is easier,
By simply setting the sample(s) to be analyzed in the autosampler, the mass information can be obtained. Monitoring a sequence analysis is also available.
*This sample was provided by the Department of Pharmaceutical Molecular Design, Meiji Pharmaceutical University.
*FIA sequence analysis such as the monitoring introduced here, the UI pad or GUI controller is required to control the HPLC module.
During a HPLC experiment, by adding the mass spectral information collected by the MS detector to the measurement data obtained by the UV (or similar) detector, the reliability of the identification result is enhanced. In addition, the SIM (selected ion monitoring) measurement improves the selectivity for the target compound. The method is particularly useful for the measurement of non-UV-absorbing samples.
By connecting the diode array detector and MS detector in series, the UV spectrum and mass spectrum can be simultaneously obtained.
With this system configuration, a large amount of sample information can be obtained. In addition, the measurement result obtained by the scanning mode of the MS detector is shown in the form of a contour display, which is commonly used for displaying data from diode array detectors. Therefore, the identity of the detected ion can be more accurately determined.
Measurement Result by Diode Array Detector
Measurement Result by 5610 MS Detector
*The data introduced here was provided by the Department of Microbial Chemistry, Kitasato University.
*These data are an example of measurement; the individual values cannot be guaranteed.
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