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    Genomic DNA Purification

    Genomic Micro AX Blood Gravity

    Genomic Micro AX Blood Gravity

    Gravity flow kit for genomic DNA purification from blood. Sample size: up to 100 µl of fresh or frozen blood.
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    Overview

    Gravity flow kit for genomic DNA purification from blood. Sample size: up to 100 µl of fresh or frozen blood.

    Item code: 101-100
    Size: 100 isolations

     

    Specifications

    Form Minicolumn
    Binding capacity 20 µg DNA
    Sample size
    • Fresh or frozen blood: up to 100 µl
    Elution volume From 120 µl
    Elution solution Elution buffer E
    Specification :
    Specifications Kindly refer to the "Overview" tab.
    Genomic-Micro-AX-Blood-Gravity-EN1.pdf
    MSDS-Genomic-Micro-AX-Blood-Gravity-EN.pdf
    Genomic Micro AX Blood Gravity
    High-Density Mapping of Quantitative Trait Loci Controlling Agronomically Important Traits in Quinoa (Chenopodium quinoa Willd.)

    Author: Nathaly Maldonado-Taipe, Federico Barbier, Karl Schmid, Christian Jung, and Nazgol Emrani

    Publication: Front Plant Sci. 2022; 13: 916067

    Quinoa is a pseudocereal originating from the Andean regions. Despite quinoa’s long cultivation history, genetic analysis of this crop is still in its infancy. We aimed to localize quantitative trait loci (QTL) contributing to the phenotypic variation of agronomically important traits. We crossed the Chilean accession PI-614889 and the Peruvian accession CHEN-109, which depicted significant differences in days to flowering, days to maturity, plant height, panicle length, and thousand kernel weight (TKW), saponin content, and mildew susceptibility. We observed sizeable phenotypic variation across F2 plants and F3 families grown in the greenhouse and the field, respectively. We used Skim-seq to genotype the F2 population and constructed a high-density genetic map with 133,923 single nucleotide polymorphism (SNPs). Fifteen QTL were found for ten traits. Two significant QTL, common in F2 and F3 generations, depicted pleiotropy for days to flowering, plant height, and TKW. The pleiotropic QTL harbored several putative candidate genes involved in photoperiod response and flowering time regulation. This study presents the first high-density genetic map of quinoa that incorporates QTL for several important agronomical traits. The pleiotropic loci can facilitate marker-assisted selection in quinoa breeding programs.

    Genomic Micro AX Blood Gravity

     
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