FM Defined Minimal Media

Overview

This media is based on well defined formulations of mineral salts, vitamins and amino acids.

Two commonly used synthetic defined minimal Dictystelium Discoideum media are FM and SIH.

FM medium developed by Franke and Kessin is used for transformation of Dictyostelium Discoideum and genomic studies. This formulation supports the growth of most strains that are capable of growing on HL5.

SIH medium is a newly developed modification of FM with regards to its amino acid compostion. Aspartic acid is added. Tryptophane and Lysine concentrations are significantly increased, resulting in an increase of cell density up to 5 x 10^7.

Both FM and SIH media are based on mineral salts in micro and macro concentrations supplemented with several vitamins. Besides these components, there is a large group of amino acids present. To facilitate genetic studies Formedium™offers complete formulations of FM and SIH as well as both media lacking various amino acids such as without Arginine, Glutamic Acid, Lysine and Methionine.

New to our range of synthetic media are FM and SIH without all amino acids. These two newly developed media combined with a range of amino acid drop-out mixtures gives the option to make every synthetic drop-out medium required for genetic studies.

Improvement of a synthetic medium for Dictyostelium Discoideum,
Sang-In Han, Karl Friehs and Erwin Flaschel,
Process Biochemistry, 39 (8), 925 – 930, 2004.

Product Range

• FM Minimal Medium Without Iron
• FM Minimal Medium Without Phosphates
• FM Minimal Medium
• FM Minimal medium w/o NH4Cl
• FM Minimal medium w/o Amino acids
• FM Minimal medium w/o Amino acids and w/o Ammonium chloride
• FM Minimal medium w/o Methionine
• FM Minimal medium w/o Arginine and w/o Lysine
• FM Minimal medium w/o Cysteine and w/o Methionine
• FM Minimal medium w/o Glutamic acid and w/o Lysine
• FM Amino acid drop-out mixtures
• FM drop-out mixture, minus Arginine, 7600 mg/l
• FM drop-out mixture, minus Cysteine, 8100 mg/l
• FM drop-out mixture, minus Glutamic acid, 7800 mg/l
• FM drop-out mixture, minus Lysine, 7400 mg/l
• FM drop-out mixture, minus Methionine, 8000 mg/l
• FM drop-out mixture, minus Arginine and w/o Cysteine, 7400 mg/l
• FM drop-out mixture, minus Arginine and w/o Glutamic acid, 7100 mg/l
• FM drop-out mixture, minus Arginine and w/o Lysine, 6700 mg/l
• FM drop-out mixture, minus Arginine and w/o Methionine, 7300 mg/l
• FM drop-out mixture, minus Cysteine and w/o Glutamic acid, 7600 mg/l
• FM drop-out mixture, minus Cysteine and w/o Lysine, 7200 mg/l
• FM drop-out mixture, minus Cysteine and w/o Methionine, 7800 mg/l
• FM drop-out mixture, minus Glutamic acid and w/o Lysine, 6900 mg/l
• FM drop-out mixture, minus Glutamic acid and w/o Methionine, 7500 mg/l
• FM drop-out mixture, minus Lysine and w/o Methionine, 7100 mg/l

For more details, kindly refer to the "Downloads" tab.