Singapore SingaporeSG China ChinaCN Malaysia MalaysiaMY Indonesia IndonesiaID Myanmar MyanmarMM Registration Log In
    • Products
      • General Laboratory
      • Life Science
      • Bio Reagents & Consumables
      • Analytical Science
      • Material Science & Nanotechnology
      • Pharmaceuticals
      • Medical
      • Safety Equipment
      • Customization Products & Turnkey Projects
    • Brands
    • Services
      • Exclusive Services
      • Customization and Fabrication
      • Environmental Monitoring Solutions - Data Loggers/ Alarms
      • Equipment Shifting/ Re-location
      • Product Sourcing
    • Contact Us
    • Home
    • About Us
    • Careers
    • Promotions
    • News & Events
    • Webinars
    • Our Network
    +65 6897 2772 / +65 6270 8884 / enquiry@gaiascience.com.sg Cart: 0

    Bio Reagents & Consumables

    1. Home
    2. Products
    3. Bio Reagents & Consumables
    4. Transfection Reagents
    5. Magnetofection
    6. FluoMag Transfection Reagent
    Back

    Magnetofection

    FluoMag Transfection Reagent

    FluoMag Transfection Reagent

    Fluorescent Magnetofection Reagents
    Pricing & Availability Download Catalogue Add to wishlist OZ Biosciences
    Brand: OZ Biosciences Available Quick Share:
    Add to Cart Contact Us for better pricing. Warranty Details
    Free delivery within Singapore only
    • Overview
    • Specification
    • Downloads
    • Publications
    • Product Images
    • Product Videos
    • Product Enquiry

    Overview

    OZ Biosciences has created fluorescently-labeled magnetic nanoparticles for Magnetofection applications. FluoMag Transfection Reagents are as efficient as their non-labeled counterparts and allow you to visualize the nanoparticles in vitro during your experiments.
    • FluoMag-C corresponding to CombiMag (transfection reagent enhancer)
    • FluoMag-P corresponding to PolyMag (DNA transfection reagent)
    • FluoMag-S corresponding to SilenceMag (siRNA delivery)
    • FluoMag-V corresponding to ViroMag (viral transduction)
    • FluoMag-N corresponding to NeuroMag (neuron transfection)
    Advantages of the Magnetofection technology
    • Easy cell labeling and tracking
    • Non toxic
    • Serum compatible
    Sizes : 100 µL
    Storage: +4°C and protect from the light
    Shipping Conditions: Room temperature
    • This reagent needs to be used with a magnetic plate
    CATALOG NUMBER UNIT SIZE
    FN10100

    FluoMag-N 100 µL

    FC10100

    FluoMag-C 100µL

    FP10100

    FluoMag-P 100µL

    FS10100

    FluoMag -S 100µL

    FV10100

    FluoMag-V 100µL

     

    Applications

    RECOMMENDED FOR:

    • Double labeling and co-localization studies using GFP or FITC labeled nucleic acids 
    • Transfection mechanisms follow (interaction with cells, intracellular pathway ...)
    • Fluorescent resonance energy transfer (FRET) assay
    • Analyze the association of vectors with the magnetic nanoparticles
     

    Results

    NIH-3T3 Transfection with FluoMag-P transfection reagent

    Figure 1: NIH-3T3 transfection with FluoMag-P and pEGFP plasmid DNA. NIH-3T3 cells (5x104 cells/well), growing on coverslips, were transfected in 24-well plates with 1 µg of pEGFP plasmid and 1µL of  FluoMag-P per well as described in the Magnetofection instruction manual. At several times post-transfection, cells were fixed, stained with DAPI to detect nucleus (blue) and observed under a fluorescent microscope equipped with a CCD camera.

    Transfection efficiency of FluoMagS transfection reagent

    Figure 2: Transfection efficiency of FluoMag-S versus SilenceMag. GFP stably transfected MDCK and HeLa cells were plated the day before transfection in a 24-well plate. Cells were then treated with SilenceMag or FluoMag-S and siRNA (targeting GFP or targeting LacZ as control) as described in the SilenceMag instruction manual. Complexes were prepared with 1 μL of SilenceMag or FluoMag-S and 10nM (67.5ng) of siRNA. Cells were then transfected in 500 μL transfection volume. GFP expression level was monitored 72h post-transfection by detection of fluorescence intensity with a fluorometer. 

    Specification :
    Technical Resources Kindly refer to the "Downloads" tab.
    FluoMag_Protocol_OZBiosciences11.pdf
    MSDS_FluoMag_C_Reagent.pdf
    MSDS_FluoMag_V_Reagent.pdf
    MSDS_FluoMag_P_Reagent.pdf
    MSDS_FluoMag_S_Reagent.pdf
    MSDS_FluoMag_N_Reagent.pdf
    CombiMag_Protocol_OZBiosciences.pdf
    PolyMag_Protocol_OZBiosciences.pdf
    ViroMag_Protocol_OZBiosciences.pdf
    NeuroMag_Protocol_OZBiosciences.pdf
    SilenceMag_Protocol_OZBiosciences.pdf
    FluoMag_OZ_Biosciences_results.pdf
    FluoMag Transfection Reagent FluoMag Transfection Reagent FluoMag Transfection Reagent FluoMag Transfection Reagent
    Dynamics of magnetic lipoplexes studied by single particle tracking in living cells

    Sauer AM., J Control Release. 2009 Jul 20;137(2):136-45.

    Magnetofection, gene delivery under the influence of a magnetic field, is a technique to increase transfection efficiency by enforcing gene vector contact with a target cell. Mechanisms of magnetic lipoplex internalization and intracellular details of magnetofection are still unknown. In this study, cellular dynamics of magnetic lipoplexes were examined in real time by means of highly sensitive dual-color fluorescence microscopy. Single particle tracking of magnetic lipoplexes provided trajectories representing the movement of the lipoplexes during internalization and subsequent intracellular processes. Magnetic lipoplexes show a three-phase behavior similar to polyplexes. During phase I lipoplexes are attached to the cell surface and show slow cooperative transport behavior. Phase II takes place inside the cell and was characterized by anomalous and confined diffusion. Phase III represented active transport along microtubules inside the cell. The majority of lipoplexes were internalized via endocytosis during phase I. On later time scales the formation of a perinuclear ring was observed. Persisting colocalization of fluid phase marker and lipoplexes after 24 h indicated slow endosomal release. In short, the internalization characteristics of magnetic lipoplexes are very similar to that of polyplexes. Furthermore our results suggest that the magnetic field induces an increased concentration of magnetic complexes on the cell surface resulting in higher transfection efficiency.

    Magnetic assistive and hydrogel technology for enhanced survival and function of neurons

    Al-Shakli AF., 2018 Keele University

    Neurons are the targets of injury and disease in many neurological conditions, and achieving neuronal survival/repair is a key goal for regenerative medicine. In this context, genetic engineering of neurons offers a platform for (i) basic research to enhance our understanding of neuronal biology in normal, disease/and injury conditions; and (ii) for regenerative medicine to enhance the functionality of neurons. Although, a wide range of attempts have been made to promote gene delivery to primary neurons, these cells are still difficult to genetically engineer, and current methods rely heavily on viral vectors which pose safety considerations. Magnetic nanoparticles (IONPs) are currently of great interest in regenerative medicine including for non-viral gene delivery by the 'magnetofection' strategy, i.e when used with applied magnetic fields. This project aimed to examine (i) the influence of two novel uniaxial and biaxial oscillating magnetic field devices on primary neuronal transfection efficiency, and (ii) examine the safety of magnetofection using histological and electrophysiological studies. In order to do this, a robust protocol to derive primary cortical neurons was first established.

    A second issue is that surgical delivery of Neurons results in low survival. Additionally, most basic research has relied on neurons grown on 'hard‘ substrates such as plastic, which do not mimic the mechanical properties of the in vivo microenvironment. To address these limitations, primary cortical neurons were grown in a 3-dimensional 'soft' collagen hydrogel construct which can serve both as a protective cell delivery system and a 'neuromimetic' substrate. The safety of the established protocol was evaluated by electrophysiological analyses on neurons.

    The findings demonstrate that the safety of magnetofection is magnetic field dependent, and at optimal conditions, electrophysiological properties of the nano-engineered neurons were normal. Secondly, I have shown that collagen hydrogels can support the 3D growth of neurons and electrophysiological studies can be carried out on the construct neurons; small differences were found between neurons grown on hard and soft materials. Finally, the amenability of genetic engineering of neurons within hydrogels using IONPs has been shown.

    FluoMag Transfection Reagent FluoMag Transfection Reagent FluoMag Transfection Reagent FluoMag Transfection Reagent

     
    Back to product list
    • Previous Product
    • Next Product

    Warranty Details

    Email


     

    Keep in touch:

    Quick Links

    • Home
    • Products
    • About Us
    • Brands
    • Careers
    • Services
    • Contact Us
    • Promotions
    • News & Events
    • Webinars
    • Our Network

    Products

    • General Laboratory
    • Life Science
    • Bio Reagents & Consumables
    • Analytical Science
    • Material Science & Nanotechnology
    • Pharmaceuticals
    • Medical
    • Safety Equipment
    • Customization Products & Turnkey Projects

    Customer Service

    • Enquiry
    • Help & FAQ
    • Terms and Conditions

    Gaia Science Pte Ltd

    Block 102 E Pasir Panjang Road
    #02-07, Singapore-118529
    +65 6897 2772 +65 6270 8884 enquiry@gaiascience.com.sg
    Copyright © 2025 Gaia Science Pte. Ltd. All Rights Reserved.
    Powered By Xsosys

    Sign In Here

    Sign In to Start Your Session

    I Forgot My Password

    Main Menu

    Products

    • General Laboratory
    • Life Science
    • Bio Reagents & Consumables
    • Analytical Science
    • Material Science & Nanotechnology
    • Pharmaceuticals
    • Medical
    • Safety Equipment
    • Customization Products & Turnkey Projects

    Quick Links

    • Home
    • Products
    • About Us
    • Brands
    • Careers
    • Services
    • Contact Us
    • Promotions
    • News & Events
    • Webinars
    • Our Network

    Customer Service

    • Enquiry
    • Help & FAQ
    • Terms and Conditions