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Bio Reagents & Consumables
ELISA (Enzyme-linked Immunosorbent Assay)
ELK1492 Human Ab1-40 (Amyloid Beta Peptide 1-40) ELISA Kit
Human ELISA Kit
Brand: ELK Biotechnology
Available
Free delivery within Singapore only
Overview
| Product name: | Human Ab1-40(Amyloid Beta Peptide 1-40) ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | Amyloid Beta Peptide 1-40 |
| Assay Type: | Sandwich |
| Sensitivity: | 4.58 pg/mL |
| Standard: | 1000 pg/mL |
| Range: | 15.63-1000 pg/mL |
| Sample Type: | Serum, plasma and other biological fluids |
| Assay Length: | 3.5h |
| Research Area: | Infection immunity;Neuro science; |
| Test principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Ab1-40. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Ab1-40. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Ab1-40, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Ab1-40 in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard curve
| Concentration (ng/mL) | OD | Corrected OD |
|---|---|---|
| 1000.00 | 2.082 | 1.987 |
| 500.00 | 1.621 | 1.526 |
| 250.00 | 0.997 | 0.902 |
| 125.00 | 0.765 | 0.670 |
| 62.50 | 0.503 | 0.408 |
| 31.25 | 0.351 | 0.256 |
| 15.63 | 0.221 | 0.126 |
| 0.00 | 0.095 | 0.000 |
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant Ab1-40 and the recovery rates were calculated by comparing the measured value to the expected amount of Ab1-40 in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 80-94% | 87% |
| EDTA plasma(n=5) | 89-101% | 95% |
| Heparin plasma(n=5) | 82-95% | 88% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ab1-40 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 89-97% | 86-102% | 94-106% | 85-97% |
| EDTA plasma(n=5) | 85-97% | 93-106% | 83-95% | 88-103% |
| Heparin plasma(n=5) | 95-103% | 88-96% | 81-95% | 91-102% |
Specification :
| Details | Kindly refer to the "Overview" tab. |
