EasyPure® HiPure Plasmid MaxiPrep Kit uses a modified alkaline lysis method to isolate high quality plasmid DNA from ≤ 500 ml (LB) of bacterial culture. Unique formulated lysis buffer and neutralization buffer permit complete bacterial cell lysis and neutralization. Endotoxin is removed by a simple incubation on column with a novel buffer. The purified DNA is suitable for a variety of molecular biology applications including restriction enzyme digestion, ligation, transformation, DNA sequencing, and transfection.
• Fast: the whole procedure can be performed in one hour.
• Simple: endotoxin is removed on column.
• High yield: DNA yield up to 1 mg.
Storage
RNase A at -20°C for two years; others at room temperature (15-25°C) for one year.
Shipping
RNase A on dry ice (-70 °C); others at room temperature.
Product Contents
| Component |
EM121-01 (10 rxns) |
| Resuspension Buffer (RB) |
120 ml |
| Lysis Buffer (LB) |
120 ml |
| Neutralization Buffer (NB) |
160 ml |
| ToxinOut Buffer (TB) |
60 ml |
| Wash Buffer (WB) |
25 ml |
| Elution Buffer (EB) |
25 ml |
| RNase A (10 mg/ml) |
1.2 ml |
| Maxi-Plasmid Spin Columns with Collection Tubes |
10 each |
SingaporeSG
ChinaCN
MalaysiaMY
IndonesiaID
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