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Bio Reagents & Consumables
Cell Detection
Comet Assay Kit (3-Well Slides)
Abbkine Comet Assay Kit (3-Well Slides) is a single cell gel electrophoresis test (SCGE), rapid and sensitive detection of cell DNA damage kit.
Brand: Abbkine
Not Available
Free delivery within Singapore only
Specifications
| Applications notes | Comet Assay Kit is a single cell gel electrophoresis assay (SCGE), rapid and sensitive test for detecting DNA damage in cells. |
Product Properties
| Kit components | • Comet Slides (3-Well) • 10× Lysis Solution • EDTA (500 mM) • Agarose (Low Melting Point) • Propidium Iodide (PI) (50×) • 40 mM Tris-HCl (pH 7.5) |
| Features & Benefits | • Complete reagents: Provide unique comet slides; • Simple operation: no need to use different melting point agarose cumbersome glue, can quickly and sensitively detect cell DNA damage; • Reliable: Compared to multilayer agaroses, the sample is not easy to fall off. |
| Usage notes | • Do not mix or substitute reagents or materials from other kit lots or vendors. |
| Storage instructions | Stored at 4°C for 6 months, protected from light |
| Shipping | Gel pack with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Principle | Comet Assay Kit (3-Well Slides) is a single cell gel electrophoresis assay (SCGE), rapid and sensitive test for detecting DNA damage in cells. The principle is that after organs or tissues have been suffered from (such as radiation, heavy metals, etc.), single strand or double strand DNA was broken in the cells, the cell is mixed with melted agarose and added on a slide, then the cell membrane is disrupted with lysis solution, the DNA is uncoiled with alkaline solution. Finally, the samples were electrophoresed, under the action of an electric field, the macromolecular DNA of normal cells migrates for a shorter distance, intact DNA remains in the range of the nucleus, broken pieces of DNA migrate out of the nucleus, stained with PI dye, observed under a fluorescence microscope, intact DNA forms a circular or slightly tailed pattern; DNA fragmentation fragments form comet-like pattern. According to the pH value of the electrophoresis buffer, it can be divided into neutral comet assay (pH=8.4) and alkaline comet assay (pH>13). Neutral comet assay was mainly used to detect the broken damage of DNA double strand, and alkaline comet assay has higher sensitivity and can be used to detect less single strand and double strand broken damage. |
Additional Information
| Background | Comet Assay Kit (3-Well Slides) is a single cell gel electrophoresis assay (SCGE), rapid and sensitive test for detecting DNA damage in cells. The principle is that after organs or tissues have been suffered from (such as radiation, heavy metals, etc.), single strand or double strand DNA was broken in the cells, the cell is mixed with melted agarose and added on a slide, then the cell membrane is disrupted with lysis solution, the DNA is uncoiled with alkaline solution. Finally, the samples were electrophoresed, under the action of an electric field, the macromolecular DNA of normal cells migrates for a shorter distance, intact DNA remains in the range of the nucleus, broken pieces of DNA migrate out of the nucleus, stained with PI dye, observed under a fluorescence microscope, intact DNA forms a circular or slightly tailed pattern; DNA fragmentation fragments form comet-like pattern. |
Compatible Products
| Brand | Catalog No. | Product Name |
| R&D Systems | 4890-050-K |
TACS MTT Cell Proliferation Assay |
Specification :
| Specifications | Kindly refer to the "Overview" tab. |
