BL21(DE3) pLysS Chemically Competent Cell is specifically designed for chemical transformation of DNA. It is resistant to chloramphenicol(CamR) and permits a transformation efficiency of over 107 cfu/μg DNA (tested by pUC19 plasmid DNA).
F-ompT hsdS(rB-mB-) gal dcm(DE3) pLysS CamR
• Transformation efficiency: >107 cfu/μg (pUC19 DNA).
• Contains pLysS plasmid that expresses the T7 lysozyme gene to reduce the background of the target protein expression without disturbing IPTG functioning.
• Suitable for non-toxic and toxic protein's expression.
• Control plasmid III (Amp+) is used for detection of expression function of cell. The protein size is about 25 kDa.
at -70°C for six months. Do not store in liquid nitrogen.