BL21(DE3) pLysS Chemically Competent Cell

BL21(DE3) pLysS Chemically Competent Cell is specifically designed for chemical transformation of DNA. It is resistant to chloramphenicol(Cam^R) and permits a transformation efficiency of over 10⁷ cfu/μg DNA (tested by pUC19 plasmid DNA).

Genotype
F^- ompT hsdS(r_B^-m_B^-) gal dcm(DE3) pLysS Cam^R

Features
• Transformation efficiency: >10⁷ cfu/μg (pUC19 DNA).
• Cam^R.
• Contains pLysS plasmid that expresses the T7 lysozyme gene to reduce the background of the target protein expression without disturbing IPTG functioning.
• Suitable for non-toxic and toxic protein's expression.
• Control plasmid III (Amp⁺) is used for detection of expression function of cell. The protein size is about 25 kDa.
 

Storage
at -70°C for six months. Do not store in liquid nitrogen.

Shipping
Dry ice (-70°C).