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Bio Reagents & Consumables
Cell Detection
Annexin V-AbFluor™ 647 Apoptosis Detection Kit (Red Fluorescence)
Specifications
| Applications notes | Abbkine Annexin V-AbFluor™ 647 Apoptosis Detection Kit contains Annexin V labeled with Abbkine proprietary far-red fluorescent dye AbFluor™ 647, which allows the identification and quantitation of apoptotic cells by flow cytometry or fluorescence microscopy. Simultaneous staining of cells with AbFluor™ 647 and propidium iodide (PI) allows the discrimination of intact cells, early apoptotic and late apoptotic or necrotic cells. |
| Alternative | Annexin 5; Annexin V; AbFluor™ 647 |
Product Properties
| Kit components | • Annexin V Binding Buffer (5x) • Annexin V- AbFluor™ 647 • Propidium Iodide (PI) |
| Features & Benefits | • Abbkine AbFluor™ 647 dye Provides the same or better fluorescence and photostability as Alexa Fluor 647, Cy5, DyLight™ 649 • Annexin V- AbFluor™ 647: Abs/Em = 650/665 nm; PI: Abs/Em = 535/617 nm (with DNA) • Suitable for flow cytometry or fluorescence microscopy |
| Storage instructions | Stable for at least 6 months at 4°C from date of shipment. Protect from light and do not freeze. |
| Shipping | Blue ice |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
Additional Information
| Background | Apoptosis is a form of programmed cell death to remove unwanted, damaged, or senescent cells from tissues. In normal cells, the negative phospholipids reside on the inner side of the cellular membrane while the outer surface of the membrane is occupied by uncharged phospholipids (PS). After a cell has entered apoptosis, the negatively charged PS are transported from the inner to the outer leaflet of the plasma membrane, thus exposing PS to the external cellular environment. The human anticoagulant, Annexin V, is a 35–36 kDa Ca2+-dependent phospholipid-binding protein that has a high affinity for PS. Annexin V labeled with a fluorophore or biotin can identify apoptotic cells by binding to PS exposed on the outer leaflet. Propidium iodide (PI) is a membrane-impermeant DNA-binding dye that is commonly used to selectively stain dead cells in a cell population. PI is excluded by live cells and early apoptotic cells, but stains necrotic and late apoptotic cells with compromised membrane integrity. PI can be excited by the 488, 532, or 546 nm laser lines, and emits red ?uorescence. |
| Alternative | Annexin 5; Annexin V; AbFluor™ 647 |
Compatible Products
| Brand | Catalog No. | Product Name |
| R&D Systems | 4830-250-K | TACS Annexin V-FITC Apoptosis Detection Kit |
| Specifications | Kindly refer to the "Overview" tab. |
Author: Liu L, Chen Y, Lin X, Wu M, Li J, Xie Q, Sferra TJ, Han Y, Liu H, Cao L, Yao M, Peng J, Shen A
Publication Name: Cancer Cell Int ResearchSquare
IF: 4.175
Colorectal cancer (CRC) is one of the most highly malignant tumors and has a complicated
pathogenesis. A preliminary study identified syntrophin beta 1 (SNTB1) as a potential oncogene in CRC. However, the clinical significance, biological function, and underlying mechanisms of SNTB1 in CRC are unknown. Thus, the present study aimed to investigate the function of SNTB1 in CRC.
Author: Zhang L, Ren CF, Yang Z
Publication Name: Journal of International Translational Medicine
IF: 0.028
Developing methods to improve the regenerative capacity of somatic stem cells (SSCs) is a major challenge in regenerative medicine. Here, we propose the forced expression of LIN28A as a method to modulate cellular metabolism, which in turn enhances self-renewal, differentiation capacities, and engraftment after transplantation of various human SSCs. Mechanistically, in undifferentiated/proliferating SSCs, LIN28A induced metabolic reprogramming from oxidative phosphorylation (OxPhos) to glycolysis by activating PDK1-mediated glycolysis-TCA/OxPhos uncoupling. Mitochondria were also reprogrammed into healthy/fused mitochondria with improved functional capacity. The reprogramming allows SSCs to undergo cell proliferation more extensively with low levels of oxidative and mitochondrial stress. When the PDK1-mediated uncoupling was untethered upon differentiation, LIN28A-SSCs differentiated more efficiently with an increase of OxPhos by utilizing the reprogrammed mitochondria. This study provides mechanistic and practical approaches of utilizing LIN28A and metabolic reprogramming in order to improve SSCs utility in regenerative medicine.