Tertiary lymphoid structures (TLSs) are ectopic lymphoid aggregates that can develop within or adjacent to tumors, but protocols that can accurately identify and characterize TLSs are lacking.
During this webinar, Dr. Jessica Da Gama Duarte, Mid-Career Postdoctoral Research Fellow from Olivia Newton-John Cancer Research Institute will present the protocol for the in situ interrogation and characterization of TLSs in human and murine tissue sections using Opal-tyramide signal amplification multiplex immunohistochemistry. This protocol enables simultaneous detection of up to 7 markers (6 antigens and a DAPI counterstain). She will also describe a grading system to identify immature and mature TLSs.
You will learn: