Saccharomyces Cerevisiae

Overview

Yeast Nitrogen Base media have been prepared according to the formulae of Wickerham (1943-51) and Burkholder (1943). Their research on the nutritional requirements of yeast strains resulted in the formulation of Yeast Nitrogen Base (YNB). YNB is a well defined composition of salts, vitamins, amino acids and a nitrogen source for a vigorous growth of Sacharomyces Cerevisiae.

Besides the original formulation of YNB, Formedium™ produces many variations of this medium without one or more components. These YNB without ‘X’ media provide the researcher the possibility to grow yeast cells in the absence of a certain component or to replace a particular component.

* Please note all formulations within this product range are based upon Yeast Nitrogen Base without Amino Acids, without Ammonium Sulphate and without the Component/Components selected.

 

Technical Data

Yeast Morphology Agar	Yeast Nitrogen Base	Yeast Potassium Nitrate Nitrogen Base	Yeast Nitrogen Base w/o Amino Acids	Yeast Nitrogen w/o Amino Acids and w/o Ammonium Sulfate	Yeast Carbon Base	Vitamin Free Yeast Base
Nitrogen Source g/l
Ammonium Sulphate	3.5	5	–	5	–	–	5
Asparagine	1.5	–	–	–	–	–	–
Potassium Nitrate	–	–	0.78	–	–	–	–
Caseine Hydrolysate	–	–	–	–	–	–	–
Carbon Source g/l
Glucose.H2O	10	–	–	–	–	10	10
Galactose	–	–	–	–	–	–	–
Raffinose	–	–	–	–	–	–	–
Amino Acids mg/l
Histidine.HCl	10	10	1	–	–	1	10
Methionine	20	20	2	–	–	2	20
Tryptophan	20	20	2	–	–	2	20
Vitamins µg/l
Biotin	2	2	2	2	2	2	–
Ca-Panthotenate	400	400	400	400	400	400	–
Folic Acid	2	2	2	2	2	2	–
Inositol	2000	2000	2000	2000	2000	2000	–
Nicotinic Acid	400	400	400	400	400	400	–
p-Aminobenzoic Acid	200	200	200	200	200	200	–
Pyridoxine HCl	400	400	400	400	400	400	–
Riboflavin	200	200	200	200	200	200	–
Thiamine HCl	400	400	400	400	400	400	–
Trace Elements µg/l
Boric Acid	500	500	500	500	500	500	500
Copper Sulfate	40	40	40	40	40	40	40
Potassium Iodide	100	100	100	100	100	100	100
Ferric Chloride	200	200	200	200	200	200	200
Manganese Sulfate	400	400	400	400	400	400	400
Sodium Molybdate	200	200	200	200	200	200	200
Zinc Sulfate	400	400	400	400	400	400	400
Minerals g/l
KH2PO4	1	1	1	1	1	1	1
Magnesium Sulphate.anh	0.5	0.5	0.5	0.5	0.5	0.5	0.5
Sodium Chloride	0.1	0.1	0.1	0.1	0.1	0.1	0.1
Calcium Chloride.anh	0.1	0.1	0.1	0.1	0.1	0.1	0.1
Agar g/l
Agar	18	–	–	–	–	–	–

 

Product Range

• Translucent K+ free medium, YNB w/o Amino acids and without Ammonium sulphate and w/o Potassium and w/o Folic acid and w/o Riboflavin
• Translucent K+ free medium”, YNB w/o Amino acids and without Ammonium sulphate and w/o Potassium
• Yeast Nitrogen Base without Amino acids and without Ammonium sulphate
• Yeast Nitrogen Base without Amino acids, without Ammonium sulphate and without para-Amino Benzoic acid
• Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Biotin
• Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Boron
• Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Calcium
• Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Calcium Pantothenate
• Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Copper
• Yeast Nitrogen base without Amino acids, without Ammonium sulphate, without Copper and without Iron
• Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Folic Acid
• Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Folic Acid and Riboflavin
• Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Inositol
• Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Iodine
• Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Iron
• Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Magnesium
• Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Manganese
• Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Molybdene
• Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Nicotinic acid
• Yeast Nitrogen Base without Amino Acids, without Ammonium sulphate and without Phosphate, supplemented with KCl
• Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Pyridoxine
• Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Riboflavin
• Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Sodium
• Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Thiamine
• Yeast Nitrogen base without Amino acids, without Ammonium sulphate and without Zinc

For more details, kindly refer to the "Downloads" tab.