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Saccharomyces Cerevisiae

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Yeast Nitrogen Base Media

YNB is a well-defined composition of salts, vitamins, amino acids and a nitrogen source for a vigorous growth of Sacharomyces Cerevisiae.

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Overview

Overview

Yeast Nitrogen Base media have been prepared according to the formulae of Wickerham (1943-51) and Burkholder (1943). Their research on the nutritional requirements of yeast strains resulted in the formulation of Yeast Nitrogen Base (YNB). YNB is a well-defined composition of salts, vitamins, amino acids and a nitrogen source for a vigorous growth of Sacharomyces Cerevisiae.

Besides the original formulation of YNB, Formedium™ produces many variations of this medium without one or more components. These YNB without ‘X’ media provide the researcher the possibility to grow yeast cells in the absence of a certain component or to replace a particular component.

 

Technical Data

Yeast Morphology Agar

Yeast Nitrogen Base

Yeast Potassium Nitrate Nitrogen Base

Yeast Nitrogen Base w/o Amino Acids

Yeast Nitrogen w/o Amino Acids and w/o Ammonium Sulfate

Yeast Carbon Base

Vitamin Free Yeast Base

Nitrogen Source g/l

 

 

 

 

 

 

 

Ammonium Sulphate

3.5

5

–

5

–

–

5

Asparagine

1.5

–

–

–

–

–

–

Potassium Nitrate

–

–

0.78

–

–

–

–

Caseine Hydrolysate

 –

–

–

–

–

–

–

Carbon Source g/l

 

 

 

 

 

 

 

Glucose.H2O

10

–

–

–

–

10

10

Galactose

–

–

–

–

–

–

–

Raffinose

–

–

–

–

–

–

–

Amino Acids mg/l

 

 

 

 

 

 

 

Histidine.HCl

10

10

1

–

–

1

10

Methionine

20

20

2

–

–

2

20

Tryptophan

20

20

2

–

–

2

20

Vitamins µg/l

 

 

 

 

 

 

 

Biotin

2

2

2

2

2

2

–

Ca-Panthotenate

400

400

400

400

400

400

–

Folic Acid

2

2

2

2

2

2

–

Inositol

2000

2000

2000

2000

2000

2000

–

Nicotinic Acid

400

400

400

400

400

400

–

p-Aminobenzoic Acid

200

200

200

200

200

200

–

Pyridoxine HCl

400

400

400

400

400

400

–

Riboflavin

200

200

200

200

200

200

–

Thiamine HCl

400

400

400

400

400

400

–

Trace Elements µg/l

 

 

 

 

 

 

 

Boric Acid

500

500

500

500

500

500

500

Copper Sulfate

40

40

40

40

40

40

40

Potsssium Iodide

100

100

100

100

100

100

100

Ferric Chloride

200

200

200

200

200

200

200

Manganese Sulfate

400

400

400

400

400

400

400

Sodium Molybdate

200

200

200

200

200

200

200

Zinc Sulfate

400

400

400

400

400

400

400

Minerals g/l

 

 

 

 

 

 

 

KH2PO4

1

1

1

1

1

1

1

Magnesium Sulphate.anh

0.5

0.5

0.5

0.5

0.5

0.5

0.5

Sodium Chloride

0.1

0.1

0.1

0.1

0.1

0.1

0.1

Calcium Chloride.anh

0.1

0.1

0.1

0.1

0.1

0.1

0.1

Agar g/l

 

 

 

 

 

 

 

Agar

18

–

–

–

–

–

–

 

Product Range

For more details, kindly refer to the "Downloads" tab.