View Enquiry List

Quick Links

Products

Customer Service

Magnetofection

Back
<
>

ViroMag R/L Transduction Reagent

For Lentivirus and Retrovirus transduction

Whatsapp

Overview

Overview

ViroMag R/L Transduction Reagent is a magnetic nanoparticles formulation optimized for Retroviruses and Lentiviruses. Based on the Magnetofection™ technology, this reagent allows concentrating the complete applied dose of Retro/Lentiviral particles onto cells within minutes, inducing a significant improvement of virus infectivity with extremely low vector doses. Due to its specific properties, ViroMag R/L is ideal to infect non permissive cells. This reagent demonstrates an exceptionally high efficiency to promote, control and assist viral transductions in a  so that no molecular biology processes or biochemical modifications are required.
  • Increase transduction efficiency
  • Accelerate the transduction process
  • Efficient for hard-to-infect and non-permissive cells
  • Concentrate the viral dose onto the cells
  • Suitable for all type of Retro/Lentivirus
  • Synchronise cells adsorption/infection without modifications of the viruses
 
"Use of ViroMag R/L to knock down a gene by CRISPR/Cas9-guided genome editing with Lentivirus in THP-1 cells"
Safronova A et al, Nature Immuno. 2018
 
"The magnetofection beads have been a game-changer for the transduction efficiency of our lentiviruses. We are using very hard to transduce B-lymphoblastomas that grow in suspension and viral concentration using filtering in conjunction with ViroMag R/L has brought our efficiency up from about 2% to 70-80%. High transduction efficiency on mammary epithelial organoids in suspension"
Erna Magnúsdóttir - University of Iceland
 
Sizes:
  • 100 µL 30-500 transductions in 96-well plate
  • 200 µL 60-1,000 transductions in 96 well plate
  • 1 mL 300-5,000 transductions in 96-well plate
  • #KC30700: ViroMag R/L Starting Kit with Super Magnetic plate + 200µL of ViroMag R/L
  • #KC30796 : ViroMag R/L Starting Kit with plate 96 magnets + 200µL of ViroMag R/L
  • #KC30600: ViroMag Triple Starting Kit with Super Magnetic plate + 100 µL of ViroMag, AdenoMag, ViroMag RL
  • #KC30696 : ViroMag Triple Starting Kit with plate 96 magnets + 100 µL of ViroMag, AdenoMag, ViroMag RL
Storage: +4°C
Shipping Conditions: Room Temperature
  • This reagent needs to be used with a magnetic plate
CATALOG NUMBER UNIT SIZE
RL40100

100 µL
RL40200

200 µL
RL41000

1 mL
KC30700

ViroMag R/L Starting Kit
KC30796

ViroMag R/L Starting kit plate 96 magnets
KC30600

ViroMag Triple Starting Kit
KC30696

ViroMag Triple Starting Kit with plate 96 magnets

 

Applications

  • Perfect for cell transduction with all retroviral and lentiviral vectors especially VSV-G pseudoviruses.

  • Suitable for mammalian cells: Cell lines, primary cells, hard to transfect.

RECOMMENDED FOR: Enhancing and synchronizing retro- and lentiviruses transductions.

 

Results

ViroMag RL Transduction results

Figure 1: ViroMag R/L is highly efficient for lentiviral infection. NIH-3T3 were infected with a lentivirus coding for GFP alone or with Polybrene, ViroMag and ViroMag R/L. Percentage of infected cells was determined 48 and 72H after infection by FACS analysis.

ViroMag RL Transduction results

Figure 2: ViroMag R/L is highly efficient in a dose dependent manner. NIH-3T3 were infected with a lentivirus coding for GFP in presence of several doses of ViroMag R/L. Percentage of infected cells was determined 48 and 72H after infection by FACS analysis.