PCR Enzymes

TransTaq^®-T DNA Polymerase is a mixture of EasyTaq^® DNA Polymerase with a proofreading 3’-5’ exonuclease. The fidelity is equal to EasyPfu DNA Polymerase. The yield is equal to that from EasyTaq^® DNA Polymerase. It is more suitable for high fidelity TA cloning.
 

• TransTaq^®-T DNA Polymerase offers 18-fold fidelity as compared to EasyTaq^® DNA Polymerase.

• Extension rate is about 1-2 kb/min.
• Template-independent“A”can be generated at the 3’end of the PCR product. PCR products can be directly cloned into pEASY^®-T vectors.
• Amplification of genomic DNA fragment up to 8 kb.
 

Applications
• Complex templates
• TA cloning
 

Storage
at -20°C for two years

 

Shipping
Dry ice (-70°C)

 

Product Contents

Component	AP122-01/11	AP122-02/12	AP122-03/13
TransTaq®-T DNA Polymerase	250 U×1	500 U×1	500 U×6
10×TransTaq®	1.2 ml ×1	1.2 ml ×1	1.2 ml ×6
2.5 mM dNTPs	- / 400 μl ×1	- / 800 μl ×1	- / 800 μl ×6
6×DNA Loading Buffer	500 μl×1	1 ml ×1	1 ml ×2