PCR Enzymes

TransTaq^® DNA Polymerase High Fidelity (TransTaq^®HiFi DNA Polymerase) contains TransTaq^®-T DNA Polymerase and a proofreading 3’-5’ exonuclease.TransTaq^® HiFi DNA Polymerase provides higher specificity and higher amplification efficiency than TransTaq^®-T DNA Polymerase. Two different buffers are provided in the kit.TransTaq^® HiFi Buffer I is optimized for the amplification of genomic DNA and TransTaq^® HiFi Buffer II is optimized for the amplification of λDNA, cDNA or plasmid DNA.
 

• TransTaq^® HiFi DNA Polymerase offers 18-fold fidelity as compared to EasyTaq^® DNA Polymerase.
• Extension rate is about 1-2 kb/min.
• Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be directly cloned into pEASY^®-T vectors.
• Amplification of genomic DNA fragment up to 15 kb.
 

Applications
• Complex templates
• GC/AT rich templates
• Long PCR
• High yield PCR
 

Storage
at -20°C for two years

 

Shipping
Dry ice (-70°C)

 

Product Contents

Component	AP131-01/11	AP131-02/12	AP131-03/13
TransTaq® HiFi DNA Polymerase	250 U×1	500 U×1	500 U×6
10×TransTaq® HiFi Buffer I	1.2 ml×1	1.2 ml×1	1.2 ml×6
10×TransTaq® HiFi Buffer II	1.2 ml×1	1.2 ml×1	1.2 ml×6
2.5 mM dNTP	-/400 μl×1	-/800 μl×1	-/800 μl×6
10×GC Enhancer	200 μl×1	400 μl×1	1 ml×1
6×DNA Loading Buffer	500 μl×1	1 ml×1	1 ml×2