PCR Enzymes

TransStart^® TopTaq DNA Polymerase is an engineered version of Taq DNA Polymerase combined with TransStart^® technique. One binding protein binds to double-strand DNA template, preventing polymerase activity at room temperature. Other two binding proteins bind primers, preventing primer-dimer formation. Blocking proteins are released from primers and templates during the initial denaturation. This double blocking method has higher efficiency than antibody based, or chemically modified hot start PCR.
 

• Compared with TransStart^® Taq DNA Polymerase, TransStart^®TopTaq DNA Polymerase has higher amplification efficiency, specificity and sensitivity.
• TransStart^® TopTaq DNA Polymerase offers 18-fold fidelity as compared to EasyTaq^® DNA Polymerase.
• The specificity is higher than antibody based or chemically modified hot start DNA polymerases.
• Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be directly cloned into pEASY^®-T vectors.
• Reduced nonspecific amplification and primer dimer formation.
• Different from Taq antibody, no risk of contamination from mammalian DNA.
• Different from chemical modification, long denaturing step is not needed.
• Amplification of genomic DNA fragment up to 15 kb.
 

Applications
• Complex templates
• GC/AT-rich templates
• Multiplex PCR
• High yield PCR
 

Storage
at -20°C for two years

 

Shipping
Dry ice (-70°C)

 

Product Contents

Component	AP151-01/11	AP151-02/12	AP151-03/13
TransStart® TopTaq DNA Polymerase	250 U×1	500 U×1	500 U×6
10×TransStart® TopTaq Buffer	1.2 ml	1.2 ml×2	1.2 ml×12
2.5 mM dNTPs	-/800 μl×1	-/800 μl×2	-/1.2 ml×8
10×GC Enhancer	200 μl×1	400 μl×1	1 ml×1
6×DNA Loading Buffer	500 μl×1	1 ml×1	1 ml×2