qPCR and qRT-PCR SuperMixes

This product is a ready-to-use qPCR cocktail. It contains a novel TransStart^® TipTaq DNA Polymerase , unique hot start reagents (DNA binding proteins combined with unique chemical), optimized double cation buffer, EvaGreen I, dNTPs, PCR Enhancer, PCR stabilizer and Passive Reference Dye I/II. qPCR SuperMix is provided at 2×concentration and can be used at 1× concentration by adding template, primer and Nuclease-free Water.

 

Highlights

• A combination of chemical blocking technique with TransStart^® hot start technique to achieve complete blocking. Compared with double blocking TransStart^® TopTaq, this method provides higher sensitivity, higher specificity, better amplification.

• Double cation (K⁺, NH₄⁺) buffer enhances specificity and reduces primer-dimer formation.

 

Storage
at -20°C away from light for two years

 

Shipping
Dry ice (-70°C)

 

Product Contents

 

Component	AQ142-11/21	AQ142-12/22	AQ142-13/23	AQ142-14/24
2×TransStart® Tip Green qPCR SuperMix (+Dye I/+Dye II)	1 ml	5×1 ml	15×1 ml	25×1 ml
Nuclease-free Water	1 ml	5 ml	3×5 ml	5×5 ml