qPCR and qRT-PCR SuperMixes

Description

TransStart^® Tip Green qPCR SuperMix is a ready-to-use qPCR cocktail. It contains a novel TransStart^® TipTaq DNA Polymerase , unique hot start reagents (DNA binding proteins combined with unique chemical), optimized double cation buffer, SYBR Green I, dNTPs, PCR Enhancerm and PCR stabilizer. qPCR SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primer, passive reference dye (optional) and ddH₂O.

Highlights

• A combination of chemical blocking technique with TransStart^® hot start technique to achieve complete blocking. Compared with double blocking TransStart^®TopTaq, this method provides higher sensitivity, higher specificity, better amplification.
• Double cation (K⁺, NH₄⁺) buffer enhances specificity and reduces primer-dimer formation.
• Passive reference dyes are provided for different qPCR instruments.

Passive Reference Dye

• Passive Reference Dye I (50×)
ABI Prism7000/7300/7700/7900, Eppendorf, ABI Step One, ABI Step One Plus
• Passive Reference Dye II (50×)
ABI Prism7500, ABI Prism7500 Fast, ABI Q6, ABI Quant Studio 6/7 Flex, ABI ViiA 7, Stratagene Mx3000/Mx3005P, Corbett Rotor Gene 3000
• No Passive Reference Dye
Roche LightCycler480, Roche Light Cycler96, MJ Research Chromo4, Opticon (II), Takara TP800, Bio-Rad iCycler iQ, iQ5, Bio-Rad CFX96, Bio-Rad C1000 Thermal Cycler, Thermo Pikoreal 96, Corbett Rotor Gene 6000, Corbett Rotor Gene G, Corbett Rotor Gene Q