PCR Enzymes

TransStart^® KD Plus DNA Polymerase is a genetically modified high fidelity DNA polymerase. This enzyme provides higher amplification capability than traditional Pfu DNA polymerase and fast amplification speed equal to Taq DNA polymerase (1 kb/min). Due to strong 3’-5’ exonuclease activity, this enzyme offers 108-fold fidelity as compared to EasyTaq^® DNA Polymerase.
 

• PCR products can be directly cloned into pEASY^®-Blunt vectors.
• Amplification of genomic DNA fragment up to 15 kb.
• Amplification of plasmid DNA fragment up to 20 kb.
 

Applications
• Fast, high specificity amplification
• High fidelity, high yield amplification
 

Storage
at -20°C for two years

 

Shipping
Dry ice (-70°C)

 

Product Contents

Component	AP301-01/11	AP301-02/12	AP301-03/13
TransStart® KD Plus DNA Polymerase	100 U×1	200 U×1	200 U×6
5×TransStart® KD Plus Buffer	1.2 ml×1	1.2 ml ×2	1.2 ml ×12
2.5 mM dNTPs	- / 500 μl×1	- / 1 ml ×1	- / 1 ml ×6
50 mM MgSO4	200 μl×1	400 μl ×1	1 ml ×1
6×DNA Loading Buffer	500 μl×1	1 ml ×1	1 ml ×2