PCR Enzymes

Description

TransStart^® FastPfu Fly DNA Polymerase is a hot start, high fidelity and high processivity DNA Polymerase. TransStart^® FastPfu Fly DNA Polymerase has an extension rate of up to 6 kb/min. Compared with TransStart^® FastPfu DNA Polymerase, TransStart^® FastPfu Fly DNA Polymerase has higher extension rate, higher fidelity, and higher amplification efficiency.

Highlights

• TransStart^® FastPfu Fly DNA Polymerase offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase.
• Extension rate is about 2-6 kb/min.
• PCR products can be directly cloned into pEASY^®-Blunt vectors.
• Amplification of genomic DNA fragment up to 15 kb.
• Amplification of plasmid DNA fragment up to 20 kb.

Applications

• High fidelity PCR
• High yield and fast PCR
• Blunt end cloning
• Site-directed mutagenesis
• Complex templates

Unit Definition

One unit of TransStart^® FastPfu Fly DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74?.

Quality Control

TransStart^® FastPfu Fly DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity; >99% homogeneous measured by SDS-PAGE. Each batch of TransStart^® FastPfu Fly DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.

Storage Buffer

50 mM Tris-HCl (pH 8.2), 0.1 mM EDTA, 1 mM DTT, Stabilizers, 50% glycerol

5×TransStart^® FastPfu Fly Buffer

100 mM Tris-SO4 pH 9.2, 50 mM (NH₄)₂SO₄, 200 mM KCl; 10 mM MgSO₄; 10% Glycerol; others