RT-PCR

Unique genomic DNA remover is combined with TransScript® First-Strand cDNA Synthesis SuperMix to achieve simultaneous genomic DNA removal and cDNA synthesis. After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85℃ for 5 seconds.
 

• Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination.
• The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR.
• cDNA up to 12 kb.
 

Applications
Multiple copy and low copy gene detection
 

Storage
at -20 ℃ for two years
 

Shipping
Dry ice (-70 ℃)

Product Composition

Component	AT311-02 (50 rxns)	AT311-03 (100 rxns)	AT311-04 (500 rxns)
TransScript® RT/RI Enzyme Mix	50 μl	100 μl	5 x 100 μl
gDNA Remover	50 μl	100 μl	5 x 100 μl
2×TS Reaction Mix	500 μl	1 ml	5 x 1 ml
Random Primer(N9) (0.1 μg/μl)	50 μl	100 μl	5 x 100 μl
Anchored Oligo(dT)18 Primer (0.5 μg/μl)	50 μl	100 μl	5 x 100 μl
RNase-free Water	500 μl	1 ml	5 ml