RT-PCR

TransScript^® II Reverse Transcriptase is a recombinant M-MLV reverse transcriptase with deficient RNase H activity and increased thermostability. The enzyme is active at up to 55°C. It provides higher specificity, higher yield and more full-length cDNA products.

• Increased thermostability for more full-length cDNA products.
• Reaction temperature at 42°C-55°C.
• Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
• Anchored Oligo(dT)20 Primer for higher yield and more full length cDNA.
• cDNA up to 15 kb.

 

Applications
• First-strand cDNA synthesis, cDNA library construction, 3’ and 5’ RACE
• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template
 

Storage
at -20°C for two years

 

Shipping
Dry ice (-70°C)

 

Product Contents

Component	AH101-02
TransScript® II RT	10000 U
10×TS II RT Buffer	100 μl
Anchored Oligo(dT)20 Primer (0.5 μg/μl)	50 μl