RT-PCR

One-Step RT-PCR combines the first-strand cDNA synthesis with PCR in the same tube to simplify reaction setup and reduce the possibility of contamination. Only gene-specific primers can be used for One-Step RT-PCR. TransScript^® II RT and TransTaq^® HiFi DNA Polymerase are used in the kit. Amplification of fragment up to 8 kb.
 

Applications
• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template
 

Storage
at -20°C for two years

 

Shipping
Dry ice (-70°C)

 

Product Contents

Component	AH411-02 (200 rxns)
TransScript® II One-Step Enzyme Mix	80 μl
2×One-Step Reaction Mix	2×1 ml
RNase-free Water	2×1 ml