RT-PCR

Unique genomic DNA remover is combined with TransScript^® II First- Strand cDNA Synthesis SuperMix to achieve simultaneous genomic DNA removal and cDNA synthesis at 42-55°C. After cDNA synthesis, gDNA remover and TransScript® II RT/RI Enzyme Mix are inactivated by heating at 85°C for 5 seconds.
 

• Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination.
• The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR.

• After reaction, heat inactivation for RT/RI and gDNA remover simultaneously can avoid damage to RNA caused by heat inactivating DNase I used for pretreatment in traditional protocol.
• Simple and fast procedure.

• cDNA up to 15 kb.
 

Applications
• cDNA library construction, 3’ and 5’ RACE
• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template
 

Storage
at -20°C for two years

 

Shipping
Dry ice (-70°C)

 

Product Contents

Component	AH311-02 (50 rxns)	AH311-03 (100 rxns)
TransScript® II RT/RI Enzyme Mix	50 μl	100 μl
gDNA Remover	50 μl	100 μl
2×TS II Reaction Mix	500 μl	1 ml
Random Primer (0.1 μg/μl)	50 μl	100 μl
Anchored Oligo(dT)20 Primer (0.5 μg/μl)	50 μl	100 μl
RNase-free Water	500 μl	1 ml