qPCR and qRT-PCR SuperMixes

TransScript^® II Green One-Step qRT-PCR SuperMix is designed for one-step qRT-PCR with high sensitivity, high synthesis efficiency and high amplification efficiency. This kit firstly synthesizes first-strand cDNA with RNA as templates using reverse gene-specific primers, and then performs qPCR with the synthesized cDNA as templates using both forward and reverse gene-specific primers to achieve one step from reverse transcription to qPCR in a single tube.

 

Highlights

• TransScript^® II Green One-Step RT/RI Enzyme Mix is used to efficiently synthesize first-strand cDNA from RNA and 2×PerfectStart^TM Green One-Step qPCR SuperMix is used for qPCR, enabling simple procedures to minimize the chance of contamination.
• High sensitivity and high specificity to ensure accurate results.

 

Applications
• High-copy and low-copy gene detection
• Detection of RNA virus or trace amounts of RNA

 

Storage
at -20°C away from light for two years

 

Shipping
Dry ice (-70°C)

 

Product Contents

Component	AQ311-01	AQ311-02
TransScript® II Green One-Step RT/ RI Enzyme Mix	40 μl	160 μl
PerfectStart® Green One-Step qPCR SuperMix (2×)	1 ml	4×1 ml
Passive Reference Dye (50×)	40 μl	160 μl
RNase-free Water	1 ml	4×1 ml