RT-PCR

TransScript^® II First-Strand cDNA Synthesis SuperMix provides all the necessary components for cDNA synthesis from total RNA or mRNA. The cDNA is efficiently synthesized by TransScript^® II RT/RI Enzyme Mix and 2×TS II Reaction Mix at 42°C-55°C, of which the optimum temperature is 50°C.

• Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis to ensure its synthesis length and yield.
• The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR.
• Anchored Oligo(dT)₂₀ Primer is specifically designed to bind to the first base next to the 5' end of Poly(A) tail of mRNA, providing higher specificity and high efficiency for first-strand cDNA synthesis.

• cDNA up to 15 kb.
 

Applications
• cDNA library construction, 3’ and 5’ RACE
• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template
 

Storage
at -20°C for two years

 

Shipping
Dry ice (-70°C)

 

Product Contents

Component	AH301-02	AH301-03
TransScript® II RT/RI Enzyme Mix	50 μl	100 μl
2×TS II Reaction Mix	500 μl	1 ml
Random Primer (N9) (0.1 μg/μl)	50 μl	100 μl
Anchored Oligo(dT)20 Primer (0.5 μg/μl)	50 μl	100 μl
RNase-free Water	500 μl	1 ml