qPCR and qRT-PCR SuperMixes

TransScript^® Green miRNA Two-Step qRT-PCR SuperMix provides all the necessary components for the detection and quantification of miRNA from total RNA, small RNA or other miRNA containing samples. TransScript^® miRNA RT Enzyme Mix (Poly(A) polymerase and reverse transcriptase are included) and 2×TS miRNA Reaction Mix are supplied to efficiently add Poly(A) tails and synthesize first-strand cDNA. PerfectStartTM Green qPCR SuperMix is used for miRNA quantification.

 

Highlights

• Optimal ratio of Poly(A) polymerase to reverse transcriptase and optimized reaction buffer, so as to ensure high transcription efficiency of miRNA.

• One-step completion of Poly(A) tail addition and first-strand cDNA synthesis in a single tube.

• High amplification efficiency, specificity and sensitivity ensured by PerfectStartTM Green qPCR SuperMix, leading to accurate data.

• Passive reference dyes compatible with different qPCR instruments (normalize the fluorescent signals between reactions).

 

Applications
Multiple copy and low copy gene detection.
 

Storage
at -20°C  in dark for two years
 

Shipping
Dry ice (-70°C)

 

Product Contents

Component	AQ202-01
TransScript® miRNA RT Enzyme Mix	20 μl
2×TS miRNA Reaction Mix	200 μl
Universal miRNA qPCR Primer (10 μM)	200 μl
2×PerfectStartTM Green qPCR SuperMix	5×1 ml
Passive Reference Dye (50×)	200 μl
RNase-free Water	1 ml