RT-PCR

TransScript^® Fly RT is generated by site mutations. It provides high affinity to RNA template with fast extension rate. The cDNA is efficiently synthesized by TransScript^® Fly RT/RI Enzyme Mix and 2×TS Fly Reaction Mix. The entire reverse transcription can be completed within 5 minutes.
 

• 5 minutes reverse transcription.
• Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
• Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
• cDNA up to 12 kb.
 

Applications
Multiple copy and low copy gene fast detection
 

Storage
at -20°C for two years

 

Shipping
Dry ice (-70°C)

 

Product Contents

Component	AF301-02	AF301-03
TransScript® Fly RT/RI Enzyme Mix	50 μl	100 μl
2×TS Fly Reaction Mix	500 μl	1 ml
Random Primer (N9) (0.1 μg/μl)	50 μl	100 μl
Anchored Oligo (dT)18 Primer (0.5 μg/μl)	50 μl	100 μl
RNase-free Water	500 μl	1 ml