RT-PCR

TransScript^® First-Strand cDNA Synthesis SuperMix provides all the necessary components for cDNA synthesis from total RNA or mRNA. The cDNA is efficiently synthesized by TransScript RT/RI Enzyme Mix and 2xTS Reaction Mix.

• Deficient RNase H activity to reduce RNA template degradation during the first-strand cDNA synthesis.
• The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR.
• Anchored Oligo(dT)18 Primer for higher yield and more full length cDNA.
• cDNA up to 12 kb.
 

Applications
Multiple copy and low copy gene detection
 

Storage
at -20°C for two years

 

Shipping
Dry ice (-70°C)

 

Product Contents

Component	AT301-02	AT301-03
TransScript® RT/RI Enzyme Mix	50 μl	100 μl
2×TS Reaction Mix	500 μl	1 ml
Random Primer (N9) (0.1 μg/μl)	50 μl	100 μl
Anchored Oligo (dT)18 Primer (0.5 μg/μl)	50 μl	100 μl
RNase-free Water	500 μl	1 ml