RT-PCR

TransScript^® All-in-One First-Strand cDNA Synthesis SuperMix for PCR provides all the necessary components for cDNA synthesis from total RNA or mRNA. The SuperMix is provided at 5× concentration and used at 1× concentration by adding RNA and H2O. The resulting cDNA are suitable for regular PCR, not for qPCR.
 

• One-tube format for simple and fast setup and reduce pipetting variability.
• The optimal ratio of oligo(dT)18 primer to random primer(N9) for PCR-ready cDNA.
• PCR-ready cDNA in 30 minutes (unsuitable for qPCR).
• cDNA up to 12 kb.
 

Applications
Multiple copy and low copy gene detection
 

Storage
at -20°C for two years

 

Shipping
Dry ice (-70°C)

 

Product Contents

Component	AT321-01 (50 rxns)
5×TransScript® All-in-One SuperMix for PCR	200 μl
RNase-free Water	1 ml