NGS Library Preparation Kit

The kit is designed to prepare CUT&Tag libraries for mammalian cells for the Illumina high-throughput sequencing  platform. The prepared libraries can be used for Single-end sequencing and Paired-end sequencing. Cleavage Under  Target & Tagmentation (CUT&Tag) is a new method for studying protein interactions with DNA. Protein A/G-Tn5  transposase fusion protein and the target protein can form an immune complex through antibody bridging. Tn5  transposase is activated in the presence of Mg2+, and so short sequences containing adapters can be inserted into  both ends of the DNA fragment that binds to the target protein. Finally, library can be constructed by PCR  amplification. The kit is suitable for DNA research on the interaction of DNA-binding proteins such as transcription  factors and co-regulators. Compared with traditional ChIP-seq, it has the advantages of more accurate and stable  protein-DNA interaction information in low input of cells.

 

Shipping: Box 1 ice bag (4℃) and Box 2 Dry ice (-70℃)

 

Storage: Box 1 is stored at 4°C for 1 year and Box 2 is stored at -20°C for 1 year

Product Composition

	Component	KP172-01 (12 rxns)	KP172-02 (48 rxns)
Box 1	ConA Magnetic Beads	120 µl	480 µl
	CUT&Tag DNA Clean Beads	1.05 ml x 2	4.2 ml x 2
Box 2	ConA Beads Binding Buffer	800 µl x 3	3.2 ml x 3
	Cell Wash Buffer	10 ml x 3	40 ml x 3
	Nuclear Extraction Buffer	6 ml	6 ml x 4
	1xPro-Wash Buffer	7 ml x 2	28 ml x 2
	pAG-Tn5	12 µl	48 µl
	Ab Enhancer	20 µl	80 µl
	Tagmentation Enhancer	20 µl	80 µl
	Binding Enhancer	180 µl	720 µl
	500 mM EDTA	30 µl	120 µl
	Lysis Enhancer	20 µl	80 µl
	Proteinase K (20 mg/ml)	20 µl	80 µl
	2xCUT&Tag Library Amplification Mix	300 µl	1.2 ml
	Protease Inhibitor	420 µl	840 µl x 2
	TE Buffer (1x)	1 ml	4 ml
	Nuclease-free Water	1 ml	4 ml