PCR Enzymes

TransFast^® Taq DNA Polymerase is an engineered version of Taq DNA Polymerase. The enzyme consists of a single polypeptide with a molecular weight of approximately 94 kDa. TransFast^® Taq DNA Polymerase has 5’-3’ DNA polymerase activity and 5’-3’ exonuclease activity. It lacks 3’-5’ exonuclease activity.
 

• Extension rate is about 6 kb/min.
• Template-independent “A” can be generated at the 3′ end of the PCR product. PCR products can be directly cloned into pEASY^®-T vectors.
• Amplification of genomic DNA fragment up to 4 kb.
 

Applications
• Routine PCR
• High throughput PCR
• Colony PCR
 

Storage
at -20 °C for two years

 

Shipping
Dry ice (-70°C)

 

Product Contents

Component	AP101-01/11	AP101-02/12
TransFast® Taq DNA Polymerase	500 U×1	500 U×6
10×TransFast® Taq Buffer	1.2 ml ×1	1.2 ml ×6
2.5 mM dNTPs	- / 800 μl ×1	- / 800 μl ×6
6×DNA Loading Buffer	1 ml×1	1 ml ×2

 

Experimental Data