Expression Competent Cells

Transetta(DE3) Chemically Competent Cell is specifically designed from imported strain for chemical transformation of DNA. It is resistant to chloramphenicol(Cam^R) and permits a transformation efficiency of over 10⁷ cfu/μg DNA (tested by pUC19 plasmid DNA).

Genotype
F^- ompT hsdSB(r_B^-m_B^-) gal dcm lacY1(DE3)pRARE(argU, argW, ileX, glyT, leuW, proL)(Cam^R)

Features
• Transformation efficiency: >10⁷ cfu/μg (pUC19 DNA).
• Cam^R.
• tRNAs for 6 rare codons AUA, AGG, AGA, CUA, CCC, GGA. Enhance the expression level of proteins in the prokaryotic system.
• Control plasmid I (Amp⁺) is used for detection of expression function of cell. The protein size is about 25 kDa.
 

Storage
at -70°C for six months. Do not store in liquid nitrogen.

Shipping
Dry ice (-70°C)